Detailed information of TA system TA288591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 6465979..6467703
• Replicon: chromosome
• Accession: NZ_LR594689
• Organism: Variovorax sp. WDL1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A2J7VM05
• Locus tag: E5P1_RS30985
• Protein ID: WP_068682620.1
• Coordinates: 6465979..6466851 (+)
• Length: 291 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: E5P1_RS30990
• Protein ID: WP_083944640.1
• Coordinates: 6466876..6467703 (+)
• Length: 276 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5P1_RS30955	6461507..6462184	+	678	WP_068682613.1	hypothetical protein	-
E5P1_RS30960	6462181..6462828	+	648	WP_068682615.1	RES family NAD+ phosphorylase	-
E5P1_RS30965	6462838..6463239	-	402	Protein_6133	substrate-binding domain-containing protein	-
E5P1_RS30970	6463409..6464383	+	975	WP_068682727.1	tripartite tricarboxylate transporter substrate binding protein	-
E5P1_RS30975	6464545..6465366	+	822	WP_068682617.1	SDR family oxidoreductase	-
E5P1_RS30980	6465404..6465958	+	555	WP_068682618.1	cupin domain-containing protein	-
E5P1_RS30985	6465979..6466851	+	873	WP_068682620.1	alpha/beta fold hydrolase	Toxin
E5P1_RS30990	6466876..6467703	+	828	WP_083944640.1	IclR family transcriptional regulator	Antitoxin
E5P1_RS30995	6467714..6468547	+	834	WP_068682622.1	SDR family oxidoreductase	-
E5P1_RS31000	6468540..6470237	+	1698	WP_102905742.1	thiamine pyrophosphate-binding protein	-
E5P1_RS31005	6470247..6471047	+	801	WP_068682624.1	aspartate dehydrogenase	-
E5P1_RS31010	6471055..6472533	+	1479	WP_068682626.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (48-282)

Antitoxin

• Transcription regulator IclR, N-terminal (17-66)

Sequences

Toxin

Protein

• Length: 291 a.a.
• Molecular weight: 29900.20 Da
• Isoelectric Point: 6.4832

>T288591 WP_068682620.1 NZ_LR594689:6465979-6466851 [Variovorax sp. WDL1]
MSDAAVIDAQPLPASELARLDARFPARSVPVQGGSLVSVRECGAGRAIVCLHGIGSGSASWLDTALLLEGEARLIAWDAP
GYGESTPLAAQAPTSRDYAQRLHGLLDALEIASCVLVGHSLGALTAAAAARPGSPLASRISKLLLISPAAGYGAASRAQS
QGRVRAERLGTLDELGIAGMAAKRSGRLVSAHASDTARQWVRWNMARLHDTGYRQAVELLCGADLLADLPPAMPVRVACG
ALDVVTTPEACAEVARQCGVPLELVPEAGHASYVERPAAVAALLRDALAD

Nucleotide

• Length: 873 bp

Antitoxin

Protein

• Length: 276 a.a.
• Molecular weight: 30434.82 Da
• Isoelectric Point: 5.7780

>AT288591 WP_083944640.1 NZ_LR594689:6466876-6467703 [Variovorax sp. WDL1]
MSNDLMEEGADRYNVPALERGLRMLCEFSRESRTLSAPELARRFDLPRSTVFRLLSTLENMGFLERAEGGRDYRLGLAVL
RLGFEYLASLELTQLGTPLLNRLCEELRTPCNLVVRDGRSIVYVAKVAPPTPFASSVSVGTRLPAHATVLGRILLEDLTL
PQLRALYPEDKLETFSPSTPKTVNELFDMVQADRERGYVLGEGFFESNISTIAAPVRDHSGHIAAALGATITSGHIDENR
MDDMVRRVRETADEISGLLNYAPASKAKVVPLRSA

Nucleotide

• Length: 828 bp

Structures

Toxin

• AlphaFold DB: A0A2J7VM05