Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 1403536..1405161 Replicon chromosome
Accession NZ_LR594689
Organism Variovorax sp. WDL1

Toxin (Protein)


Gene name hipA Uniprot ID A0A2J7VX00
Locus tag E5P1_RS06950 Protein ID WP_068685242.1
Coordinates 1403812..1405161 (+) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A2J7VX08
Locus tag E5P1_RS06945 Protein ID WP_068685244.1
Coordinates 1403536..1403808 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5P1_RS06925 1399227..1400312 - 1086 WP_068685251.1 PA0069 family radical SAM protein -
E5P1_RS06930 1400416..1401315 + 900 WP_068685344.1 NAD(P)H-binding protein -
E5P1_RS06935 1401325..1401987 - 663 WP_068685249.1 glutathione S-transferase -
E5P1_RS06940 1402103..1403320 + 1218 WP_068685246.1 polyhydroxyalkanoate depolymerase -
E5P1_RS06945 1403536..1403808 + 273 WP_068685244.1 helix-turn-helix domain-containing protein Antitoxin
E5P1_RS06950 1403812..1405161 + 1350 WP_068685242.1 type II toxin-antitoxin system HipA family toxin Toxin
E5P1_RS06955 1405201..1405650 - 450 WP_068685240.1 CBS domain-containing protein -
E5P1_RS06960 1405795..1407105 + 1311 WP_068685238.1 O-acetylhomoserine aminocarboxypropyltransferase -
E5P1_RS06965 1407105..1407917 + 813 WP_068685236.1 alpha/beta hydrolase -
E5P1_RS06970 1407968..1408405 - 438 WP_068685234.1 thioredoxin family protein -
E5P1_RS06975 1408386..1409615 - 1230 WP_068685232.1 YihY family inner membrane protein -
E5P1_RS06980 1409670..1410080 + 411 WP_068685230.1 DUF2069 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(10-112)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-67)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 49993.34 Da        Isoelectric Point: 7.2139

>T288587 WP_068685242.1 NZ_LR594689:1403812-1405161 [Variovorax sp. WDL1]
MGRRSHTQALALWMNGARVGTWRIHPQAGDVLEYSPDWTASEQGRPLSLSLPFMPGGAPHRGERVRTYFENLLPDSDDIR
ARIARRYQTKSTDAFPLLAEIGRDCVGALQILPDGEVPAGVQPVQASPLNESMVAQVLRNTVSPTLVSLGDDDDTFRISI
AGAQEKTALLRFEGKWCMPHGDTPTTHIFKLPLGLIGGMKYDMSDSVENEWLCSLILKAYGLPVAACEPLQFEDVKVLAV
ERFDRSWWKSPAGDMRLIRLPQEDMCQAKGVSPHAKYEADGGPGIDSIMDVLEGSLSRQDDKRAFFQAQLVFWLLCATDG
HAKNFSLFLRPGGRYQLTPLYDVLSAYPVLGEGPGKLSPFKARMAMAIRSRNAHWKMRDILRRHWLVMGERHGVTTPDGR
DVRFVIDDLADRTPKVIRQVRAKLPKDFPEPLVEAILGGLQEAANKISD

Download         Length: 1350 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9799.38 Da        Isoelectric Point: 10.1833

>AT288587 WP_068685244.1 NZ_LR594689:1403536-1403808 [Variovorax sp. WDL1]
MPEFVLRTSEQLPGLLQAFRKQTGMTQAEAAVRLGVTQQTMSALERNAEKVSAARLMKLLSILGVELVLRKDDAMAPPKS
ASATDSNPTW

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J7VX00

Antitoxin

Source ID Structure
AlphaFold DB A0A2J7VX08

References