Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) FicTA/Fic(toxin)
Location 3120..3913 Replicon plasmid 5
Accession NZ_LR594670
Organism Variovorax sp. SRS16

Toxin (Protein)


Gene name VbhT Uniprot ID Q6QHR6
Locus tag E5P2_RS37305 Protein ID WP_011171676.1
Coordinates 3305..3913 (+) Length 203 a.a.

Antitoxin (Protein)


Gene name VbhA Uniprot ID A0A8B6LZM3
Locus tag E5P2_RS37300 Protein ID WP_011171677.1
Coordinates 3120..3308 (+) Length 63 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5P2_RS37285 1..774 + 774 WP_162572150.1 ParA family protein -
E5P2_RS37290 771..1844 + 1074 WP_011171679.1 ParB/RepB/Spo0J family partition protein -
E5P2_RS37295 2039..3055 + 1017 WP_011171678.1 DNA-binding protein -
E5P2_RS37300 3120..3308 + 189 WP_011171677.1 antitoxin VbhA family protein Antitoxin
E5P2_RS37305 3305..3913 + 609 WP_011171676.1 Fic/DOC family protein Toxin
E5P2_RS37310 3930..4274 + 345 WP_015063536.1 hypothetical protein -
E5P2_RS37315 4326..4955 + 630 WP_162572165.1 hypothetical protein -
E5P2_RS37320 5026..5463 - 438 WP_011171734.1 conjugal transfer protein TraM -
E5P2_RS37325 5463..6188 - 726 WP_011171733.1 conjugal transfer protein TraL -
E5P2_RS37330 6188..6586 - 399 WP_011171732.1 TraK family protein -
E5P2_RS37335 7011..7367 + 357 WP_162572166.1 conjugal transfer transcriptional regulator TraJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..71057 71057


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(55-150)

Antitoxin

Antitoxin VbhA

(10-56)


Sequences

Toxin        


Download         Length: 203 a.a.        Molecular weight: 22398.22 Da        Isoelectric Point: 7.4661

>T288580 WP_011171676.1 NZ_LR594670:3305-3913 [Variovorax sp. SRS16]
MKYAGDRGDPYLDSETGVLRNLLGIKEQGGLDKAESTLSFLRASELREQPVKGKFDLAHLQRIHKRLFGDVYDWAGQIRQ
VEISKGSTMFARQVAIQSAAQQLFGQLAKEQLLRGLDADEFSKRAGHYLGEINVLHPFREGNGRTQREFIGQLAQQAGHR
IDWSGVSQASMTQASIEAYNGDSSGMAGLIRAGMPDQLFFNP

Download         Length: 609 bp

>T288580 NZ_LR594670:3305-3913 [Variovorax sp. SRS16]
ATGAAATACGCCGGGGATCGCGGCGATCCTTACCTGGACAGCGAAACGGGTGTTCTCCGCAATCTCCTTGGAATCAAGGA
ACAGGGTGGACTCGATAAAGCCGAATCCACCCTTTCCTTTTTGCGGGCCAGCGAATTGCGCGAGCAGCCCGTTAAAGGCA
AATTCGATTTAGCGCACCTGCAGCGAATTCACAAGCGCCTTTTTGGCGACGTGTACGACTGGGCGGGCCAAATCCGCCAG
GTCGAAATCTCGAAAGGCAGCACCATGTTTGCCCGGCAGGTTGCGATCCAGAGCGCGGCGCAGCAGCTCTTTGGGCAGCT
TGCCAAGGAACAGCTCTTGCGCGGCCTCGATGCCGACGAGTTCAGCAAGCGGGCCGGCCACTATCTCGGGGAAATCAACG
TGCTTCATCCCTTCCGTGAAGGGAACGGCAGGACGCAGCGTGAGTTCATCGGGCAACTGGCCCAGCAGGCGGGCCACAGG
ATCGACTGGAGCGGGGTCAGTCAGGCCAGCATGACCCAGGCGTCAATCGAGGCCTACAACGGCGACTCAAGCGGTATGGC
TGGCCTTATTCGCGCTGGCATGCCAGACCAGCTTTTTTTTAACCCATGA

Antitoxin


Download         Length: 63 a.a.        Molecular weight: 6904.79 Da        Isoelectric Point: 4.4982

>AT288580 WP_011171677.1 NZ_LR594670:3120-3308 [Variovorax sp. SRS16]
MIAEQEQTERRAVVQSALASQRIEGLEPDAQAVADAERWARGEMTIGAAVDQYKARMRLEMA

Download         Length: 189 bp

>AT288580 NZ_LR594670:3120-3308 [Variovorax sp. SRS16]
ATGATCGCAGAGCAGGAACAGACCGAGCGCCGCGCCGTGGTGCAAAGCGCCCTTGCGAGCCAGCGCATCGAGGGCTTAGA
GCCCGATGCGCAGGCCGTGGCCGACGCCGAGCGCTGGGCGCGTGGCGAGATGACCATCGGTGCCGCCGTGGACCAGTACA
AGGCGCGCATGCGGCTAGAAATGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B6M0K6

Antitoxin

Source ID Structure
AlphaFold DB A0A8B6LZM3

References