Detailed information of TA system TA288579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 548483..549942
• Replicon: plasmid 3
• Accession: NZ_LR594668
• Organism: Variovorax sp. SRS16

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: E5P2_RS34865
• Protein ID: WP_162571608.1
• Coordinates: 549097..549942 (+)
• Length: 282 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: E5P2_RS34860
• Protein ID: WP_162571607.1
• Coordinates: 548483..549100 (+)
• Length: 206 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5P2_RS34840	546006..546317	-	312	WP_162571605.1	conjugal transfer protein TrbD	-
E5P2_RS34845	546321..546719	-	399	WP_197908652.1	TrbC/VirB2 family protein	-
E5P2_RS34850	546732..547700	-	969	WP_162571658.1	P-type conjugative transfer ATPase TrbB	-
E5P2_RS34855	547871..548233	-	363	WP_162571606.1	transcriptional regulator	-
E5P2_RS34860	548483..549100	+	618	WP_162571607.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
E5P2_RS34865	549097..549942	+	846	WP_162571608.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
E5P2_RS34870	550238..551269	+	1032	WP_162571609.1	site-specific integrase	-
E5P2_RS34875	551612..552157	+	546	WP_197908655.1	H-NS histone family protein	-
E5P2_RS34880	552389..552778	-	390	WP_162571610.1	hypothetical protein	-
E5P2_RS34885	552815..553153	-	339	WP_162571611.1	hypothetical protein	-
E5P2_RS34890	553213..553374	+	162	WP_162571612.1	hypothetical protein	-
E5P2_RS34895	553409..553735	-	327	WP_162571613.1	hypothetical protein	-
E5P2_RS34900	554162..554578	+	417	WP_162571614.1	hypothetical protein	-
E5P2_RS34905	554563..554832	+	270	WP_162571615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..560632	560632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (14-58)

Sequences

Toxin

Protein

• Length: 282 a.a.
• Molecular weight: 31116.70 Da
• Isoelectric Point: 6.7583

>T288579 WP_162571608.1 NZ_LR594668:549097-549942 [Variovorax sp. SRS16]
MSRNLAASILSRLKQRADASRQDFNLTLTHYGLERLLYRLSVSPHAPNYLLKGALLFSLWYDQPHRPTRDADLLGFGPDD
IDSAVSAFREICEITVEDGIAFDPASVKGSVIRKEAGYGGVRIDLQAKLDGARIALQVDIGFGDVVTPVAESVSYPVLLE
GLPAPHLRTYPKYTVVAEKFHAVCLLGMVNTRMKDYFDLWVLLHEGTLDTTELRRAIEATFARRKLALPSTLPSGLSDAF
AQDAAKQRQWAAFLQKNRLEALDLAEVVAFLRGEFEKLGAA

Nucleotide

• Length: 846 bp

Antitoxin

Protein

• Length: 206 a.a.
• Molecular weight: 22436.07 Da
• Isoelectric Point: 9.5538

>AT288579 WP_162571607.1 NZ_LR594668:548483-549100 [Variovorax sp. SRS16]
MALRSESTLEQAALALAERQPLLRARDLAEHGVATMVLSRLVSAGKLERVARGIYSLPGRALSEHRSLAEVALRVPRGVV
CLLSALRVHDIGTQAPFEVWMAIPHHSPTPRLDQPALRVVRMSGAALTEGIEPVVIDGVNVAVFNAAKTVADCFKYRNKI
GLDVALEALRDGWSQRKLTMDALWHYATADRVANVMRPYLESITA

Nucleotide

• Length: 618 bp