Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) FicTA/Fic(toxin)
Location 3120..3913 Replicon plasmid 4
Accession NZ_LR594665
Organism Variovorax sp. RA8

Toxin (Protein)


Gene name VbhT Uniprot ID Q6QHR6
Locus tag E5P3_RS35320 Protein ID WP_011171676.1
Coordinates 3305..3913 (+) Length 203 a.a.

Antitoxin (Protein)


Gene name VbhA Uniprot ID A0A8B6LZM3
Locus tag E5P3_RS35315 Protein ID WP_011171677.1
Coordinates 3120..3308 (+) Length 63 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5P3_RS35300 1..774 + 774 WP_162572150.1 ParA family protein -
E5P3_RS35305 771..1844 + 1074 WP_011171679.1 ParB/RepB/Spo0J family partition protein -
E5P3_RS35310 2039..3055 + 1017 WP_011171678.1 DNA-binding protein -
E5P3_RS35315 3120..3308 + 189 WP_011171677.1 antitoxin VbhA family protein Antitoxin
E5P3_RS35320 3305..3913 + 609 WP_011171676.1 Fic/DOC family protein Toxin
E5P3_RS35325 3930..4274 + 345 WP_015063536.1 hypothetical protein -
E5P3_RS35330 4326..4955 + 630 WP_162572165.1 hypothetical protein -
E5P3_RS35335 5026..5463 - 438 WP_011171734.1 conjugal transfer protein TraM -
E5P3_RS35340 5463..6188 - 726 WP_011171733.1 conjugal transfer protein TraL -
E5P3_RS35345 6188..6586 - 399 WP_011171732.1 TraK family protein -
E5P3_RS35350 7011..7367 + 357 WP_162572166.1 conjugal transfer transcriptional regulator TraJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..68393 68393


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(55-150)

Antitoxin

Antitoxin VbhA

(10-56)


Sequences

Toxin        


Download         Length: 203 a.a.        Molecular weight: 22398.22 Da        Isoelectric Point: 7.4661

>T288573 WP_011171676.1 NZ_LR594665:3305-3913 [Variovorax sp. RA8]
MKYAGDRGDPYLDSETGVLRNLLGIKEQGGLDKAESTLSFLRASELREQPVKGKFDLAHLQRIHKRLFGDVYDWAGQIRQ
VEISKGSTMFARQVAIQSAAQQLFGQLAKEQLLRGLDADEFSKRAGHYLGEINVLHPFREGNGRTQREFIGQLAQQAGHR
IDWSGVSQASMTQASIEAYNGDSSGMAGLIRAGMPDQLFFNP

Download         Length: 609 bp

Antitoxin


Download         Length: 63 a.a.        Molecular weight: 6904.79 Da        Isoelectric Point: 4.4982

>AT288573 WP_011171677.1 NZ_LR594665:3120-3308 [Variovorax sp. RA8]
MIAEQEQTERRAVVQSALASQRIEGLEPDAQAVADAERWARGEMTIGAAVDQYKARMRLEMA

Download         Length: 189 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B6M0K6

Antitoxin

Source ID Structure
AlphaFold DB A0A8B6LZM3

References