Detailed information of TA system TA288567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 3289598..3291325
• Replicon: chromosome
• Accession: NZ_LR594662
• Organism: Variovorax sp. RA8

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: E5P3_RS15390
• Protein ID: WP_162586763.1
• Coordinates: 3289598..3290470 (+)
• Length: 291 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: E5P3_RS15395
• Protein ID: WP_162586764.1
• Coordinates: 3290498..3291325 (+)
• Length: 276 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5P3_RS15365	3285498..3286175	+	678	WP_162586759.1	hypothetical protein	-
E5P3_RS15370	3286172..3286819	+	648	WP_162586760.1	RES family NAD+ phosphorylase	-
E5P3_RS15375	3287029..3288003	+	975	WP_162589699.1	tripartite tricarboxylate transporter substrate binding protein	-
E5P3_RS15380	3288166..3288987	+	822	WP_162586761.1	SDR family oxidoreductase	-
E5P3_RS15385	3289023..3289577	+	555	WP_162586762.1	cupin domain-containing protein	-
E5P3_RS15390	3289598..3290470	+	873	WP_162586763.1	alpha/beta hydrolase	Toxin
E5P3_RS15395	3290498..3291325	+	828	WP_162586764.1	IclR family transcriptional regulator	Antitoxin
E5P3_RS15400	3291336..3292169	+	834	WP_162586765.1	SDR family oxidoreductase	-
E5P3_RS15405	3292162..3293859	+	1698	WP_162586766.1	thiamine pyrophosphate-binding protein	-
E5P3_RS15410	3293869..3294669	+	801	WP_162586767.1	aspartate dehydrogenase	-
E5P3_RS15415	3294677..3296152	+	1476	WP_162586768.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (48-282)

Antitoxin

• Transcription regulator IclR, C-terminal (137-256)

Sequences

Toxin

Protein

• Length: 291 a.a.
• Molecular weight: 29866.27 Da
• Isoelectric Point: 6.4881

>T288567 WP_162586763.1 NZ_LR594662:3289598-3290470 [Variovorax sp. RA8]
MSDAAVIDAQPLPASELARLDARFPARSVRVQGGAVVSVRECGAGPAIVCLHGIGSGSASWLDTALLLEGEARLIAWDAP
GYGESTPLAAQAPTAHDYAQRLHGLLDALEISSCVLVGHSLGALMAASAAHPGSPLAPRISTLVLISPAAGYGAAGRAEA
RARVRAERLGTLDELGIAGMAAKRSGRLVSAHASETARQWVRWNMARLHETGYRQAVELLCGADLRADLPPAVPVRVACG
ALDVVTTPEACAEVARLCAVPLELLPEAGHASYVERPAAVAALLREAIAA

Nucleotide

• Length: 873 bp

Antitoxin

Protein

• Length: 276 a.a.
• Molecular weight: 30466.88 Da
• Isoelectric Point: 5.7806

>AT288567 WP_162586764.1 NZ_LR594662:3290498-3291325 [Variovorax sp. RA8]
MSNDLMEEGADRYNVPALERGLRMLCEFSRESRTLSAPEMARRFDLPRSTVFRLLSTLENMGFLERAEGGRDYRLGLAVL
RLGFEYLASLELTQLGTPLLNRLCEELRTPCNLVVRDGRSIVYVAKVAPPTPFASSVSVGTRLPAHATVLGRILLEDLTL
PQLRALYPEDKLETFSPSTPKTVNELFDMVQADRERGYVLGEGFFESNISTIAAPVRDHSGHIAAALGATITSGHIDENR
MDEMVRRVRETADEISGLLNYAPASKAKVVPLRSA

Nucleotide

• Length: 828 bp