Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 232948..233590 Replicon chromosome
Accession NC_020238
Organism Bacillus thuringiensis serovar kurstaki str. HD73

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag HD73_RS01315 Protein ID WP_000635965.1
Coordinates 233240..233590 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag HD73_RS01310 Protein ID WP_000004570.1
Coordinates 232948..233235 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HD73_RS01285 228265..229227 + 963 WP_000961167.1 UV DNA damage repair endonuclease UvsE -
HD73_RS01290 229220..229792 - 573 WP_000908522.1 rhomboid family intramembrane serine protease -
HD73_RS01295 229886..230245 + 360 WP_000583417.1 holo-ACP synthase -
HD73_RS01300 230402..231352 + 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
HD73_RS01305 231470..232639 + 1170 WP_000390616.1 alanine racemase -
HD73_RS01310 232948..233235 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
HD73_RS01315 233240..233590 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HD73_RS01320 233658..235826 + 2169 WP_000426236.1 RNA-binding transcriptional accessory protein -
HD73_RS01325 235885..236001 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
HD73_RS01330 236197..236655 + 459 WP_000344239.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T28855 WP_000635965.1 NC_020238:233240-233590 [Bacillus thuringiensis serovar kurstaki str. HD73]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T28855 NC_020238:233240-233590 [Bacillus thuringiensis serovar kurstaki str. HD73]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT28855 WP_000004570.1 NC_020238:232948-233235 [Bacillus thuringiensis serovar kurstaki str. HD73]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT28855 NC_020238:232948-233235 [Bacillus thuringiensis serovar kurstaki str. HD73]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References