Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4524591..4525401 Replicon chromosome
Accession NZ_LR588412
Organism Klebsiella pneumoniae strain NCTC9157

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag FGL27_RS22190 Protein ID WP_002887280.1
Coordinates 4524591..4525124 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag FGL27_RS22195 Protein ID WP_002887278.1
Coordinates 4525135..4525401 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGL27_RS22185 4523422..4524543 + 1122 WP_002887282.1 cupin domain-containing protein -
FGL27_RS22190 4524591..4525124 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
FGL27_RS22195 4525135..4525401 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
FGL27_RS22200 4525504..4526937 - 1434 WP_015959366.1 Cu(+)/Ag(+) sensor histidine kinase -
FGL27_RS22205 4526927..4527610 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
FGL27_RS22210 4527783..4529168 + 1386 WP_032428115.1 efflux transporter outer membrane subunit -
FGL27_RS22215 4529186..4529530 + 345 WP_015959364.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T288459 WP_002887280.1 NZ_LR588412:c4525124-4524591 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT288459 WP_002887278.1 NZ_LR588412:c4525401-4525135 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References