Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4572339..4573149 Replicon chromosome
Accession NZ_LR588408
Organism Klebsiella pneumoniae strain NCTC11698

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag FGL30_RS22270 Protein ID WP_004178461.1
Coordinates 4572339..4572872 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag FGL30_RS22275 Protein ID WP_002887278.1
Coordinates 4572883..4573149 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGL30_RS22265 4571170..4572291 + 1122 WP_002887282.1 cupin domain-containing protein -
FGL30_RS22270 4572339..4572872 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
FGL30_RS22275 4572883..4573149 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
FGL30_RS22280 4573252..4574685 - 1434 WP_040242656.1 Cu(+)/Ag(+) sensor histidine kinase -
FGL30_RS22285 4574675..4575358 - 684 WP_038808523.1 copper response regulator transcription factor CusR -
FGL30_RS22290 4575530..4576915 + 1386 WP_040242658.1 efflux transporter outer membrane subunit -
FGL30_RS22295 4576933..4577277 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T288436 WP_004178461.1 NZ_LR588408:c4572872-4572339 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT288436 WP_002887278.1 NZ_LR588408:c4573149-4572883 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References