Detailed information of TA system TA288422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 939551..940788
• Replicon: chromosome
• Accession: NZ_LR536843
• Organism: Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A098ZIW8
• Locus tag: E0F34_RS05120
• Protein ID: WP_000405362.1
• Coordinates: 940027..940788 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: E0F34_RS05115
• Protein ID: WP_000579613.1
• Coordinates: 939551..940027 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0F34_RS05095	934624..935478	+	855	WP_000900020.1	ADP-ribosylglycohydrolase family protein	-
E0F34_RS05100	935590..936474	+	885	WP_000710119.1	diacylglycerol kinase family lipid kinase	-
E0F34_RS05105	936637..938376	+	1740	WP_162499504.1	glycoside hydrolase family 13 protein	-
E0F34_RS05110	938651..939106	+	456	WP_000500471.1	hypothetical protein	-
E0F34_RS05115	939551..940027	+	477	WP_000579613.1	helix-turn-helix domain-containing protein	Antitoxin
E0F34_RS05120	940027..940788	+	762	WP_000405362.1	toxin PezT	Toxin
E0F34_RS05125	940816..941592	+	777	Protein_962	hypothetical protein	-
E0F34_RS05130	941520..941972	+	453	WP_001808559.1	hypothetical protein	-
E0F34_RS05135	942791..943150	+	360	WP_000003750.1	hypothetical protein	-
E0F34_RS05140	943160..943525	+	366	WP_061638901.1	MobC family plasmid mobilization relaxosome protein	-
E0F34_RS05145	943512..945378	+	1867	Protein_966	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	924907..952346	27439

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29129.16 Da
• Isoelectric Point: 7.1308

>T288422 WP_000405362.1 NZ_LR536843:940027-940788 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18249.67 Da
• Isoelectric Point: 4.3462

>AT288422 WP_000579613.1 NZ_LR536843:939551-940027 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

Structures

Toxin

• AlphaFold DB: A0A098ZIW8