Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4079150..4079829 Replicon chromosome
Accession NZ_LR536431
Organism Escherichia coli isolate f9610206-5e81-11e8-bf7f-3c4a9275d6c8

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag E4Z82_RS20450 Protein ID WP_003031349.1
Coordinates 4079488..4079829 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag E4Z82_RS20445 Protein ID WP_003031347.1
Coordinates 4079150..4079467 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z82_RS20410 4074395..4075507 + 1113 WP_000005560.1 His-Xaa-Ser system radical SAM maturase HxsC -
E4Z82_RS20415 4075504..4076139 + 636 WP_001446059.1 His-Xaa-Ser repeat protein HxsA -
E4Z82_RS20420 4076285..4077445 - 1161 WP_023063357.1 IS30 family transposase -
E4Z82_RS20425 4077744..4077935 + 192 Protein_3932 DUF905 domain-containing protein -
E4Z82_RS20430 4077949..4078410 + 462 WP_003031344.1 antirestriction protein -
E4Z82_RS20435 4078426..4078902 + 477 WP_003031345.1 RadC family protein -
E4Z82_RS20440 4078911..4079132 + 222 WP_003031346.1 DUF987 domain-containing protein -
E4Z82_RS20445 4079150..4079467 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
E4Z82_RS20450 4079488..4079829 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
E4Z82_RS20460 4080409..4081662 - 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
E4Z82_RS20465 4081674..4082777 - 1104 WP_001285288.1 glutamate 5-kinase -
E4Z82_RS20470 4083065..4084120 + 1056 WP_000749861.1 phosphoporin PhoE -
E4Z82_RS20475 4084159..4084560 - 402 WP_000174677.1 sigma factor-binding protein Crl -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T288417 WP_003031349.1 NZ_LR536431:4079488-4079829 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT288417 WP_003031347.1 NZ_LR536431:4079150-4079467 [Escherichia coli]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References