Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3614866..3615545 Replicon chromosome
Accession NZ_LR536430
Organism Escherichia coli isolate f974b26a-5e81-11e8-bf7f-3c4a9275d6c8

Toxin (Protein)


Gene name ykfI Uniprot ID S1FHS4
Locus tag E4Z69_RS17995 Protein ID WP_000854680.1
Coordinates 3614866..3615207 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag E4Z69_RS18000 Protein ID WP_000070396.1
Coordinates 3615228..3615545 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z69_RS17980 3610642..3611762 + 1121 WP_087451024.1 IS3-like element ISSen4 family transposase -
E4Z69_RS17985 3611844..3612263 + 420 WP_001333214.1 hypothetical protein -
E4Z69_RS17990 3612579..3614045 + 1467 WP_000723924.1 hypothetical protein -
E4Z69_RS17995 3614866..3615207 - 342 WP_000854680.1 type IV toxin-antitoxin system toxin YkfI Toxin
E4Z69_RS18000 3615228..3615545 - 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
E4Z69_RS18005 3615564..3615785 - 222 WP_000691994.1 DUF987 domain-containing protein -
E4Z69_RS18010 3615794..3616270 - 477 WP_001549015.1 RadC family protein -
E4Z69_RS18015 3616286..3616744 - 459 WP_000211838.1 antirestriction protein -
E4Z69_RS18020 3616842..3617081 - 240 WP_000194654.1 DUF905 domain-containing protein -
E4Z69_RS18025 3617158..3617625 - 468 WP_001385283.1 hypothetical protein -
E4Z69_RS18030 3617648..3618091 - 444 WP_000649865.1 hypothetical protein -
E4Z69_RS18035 3618091..3618327 - 237 WP_001144031.1 hypothetical protein -
E4Z69_RS18040 3618368..3619069 - 702 WP_000189411.1 WYL domain-containing protein -
E4Z69_RS18045 3619286..3620107 - 822 WP_000197388.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3574627..3643206 68579


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12892.95 Da        Isoelectric Point: 9.6543

>T288399 WP_000854680.1 NZ_LR536430:c3615207-3614866 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT288399 WP_000070396.1 NZ_LR536430:c3615545-3615228 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHS4

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References