Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3200850..3201426 Replicon chromosome
Accession NZ_LR536430
Organism Escherichia coli isolate f974b26a-5e81-11e8-bf7f-3c4a9275d6c8

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag E4Z69_RS15915 Protein ID WP_001297643.1
Coordinates 3200850..3201137 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag E4Z69_RS15920 Protein ID WP_000063160.1
Coordinates 3201124..3201426 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z69_RS15900 3197087..3198367 - 1281 WP_001037390.1 DUF445 domain-containing protein -
E4Z69_RS15905 3198408..3199640 - 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
E4Z69_RS15910 3199955..3200651 + 697 Protein_3061 IS1 family transposase -
E4Z69_RS15915 3200850..3201137 + 288 WP_001297643.1 BrnT family toxin Toxin
E4Z69_RS15920 3201124..3201426 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
E4Z69_RS15925 3201460..3202416 - 957 WP_001297640.1 GTPase -
E4Z69_RS15930 3202427..3202630 - 204 WP_000467859.1 YbdD/YjiX family protein -
E4Z69_RS15935 3202680..3204830 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3200442..3200651 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T288396 WP_001297643.1 NZ_LR536430:3200850-3201137 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT288396 WP_000063160.1 NZ_LR536430:3201124-3201426 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References