Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 334544..335182 Replicon chromosome
Accession NZ_LR536430
Organism Escherichia coli isolate f974b26a-5e81-11e8-bf7f-3c4a9275d6c8

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag E4Z69_RS01765 Protein ID WP_000813794.1
Coordinates 334544..334720 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag E4Z69_RS01770 Protein ID WP_001270286.1
Coordinates 334766..335182 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z69_RS01745 330163..331377 - 1215 WP_001324881.1 BenE family transporter YdcO -
E4Z69_RS01750 331430..331966 + 537 WP_000429169.1 DNA-binding transcriptional regulator SutR -
E4Z69_RS01755 332039..334000 + 1962 WP_134393380.1 23S rRNA 5-hydroxycytidine C2501 synthase -
E4Z69_RS01760 334092..334322 - 231 WP_000494244.1 YncJ family protein -
E4Z69_RS01765 334544..334720 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
E4Z69_RS01770 334766..335182 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
E4Z69_RS01775 335261..336667 + 1407 WP_000760630.1 PLP-dependent aminotransferase family protein -
E4Z69_RS01780 336912..338057 + 1146 WP_000047430.1 ABC transporter substrate-binding protein -
E4Z69_RS01785 338075..339088 + 1014 WP_000220434.1 ABC transporter ATP-binding protein -
E4Z69_RS01790 339089..340030 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T288380 WP_000813794.1 NZ_LR536430:334544-334720 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T288380 NZ_LR536430:334544-334720 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT288380 WP_001270286.1 NZ_LR536430:334766-335182 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT288380 NZ_LR536430:334766-335182 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References