Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4633933..4634430 Replicon chromosome
Accession NZ_LR217818
Organism Escherichia coli strain NCTC86

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag E4V11_RS22695 Protein ID WP_001173343.1
Coordinates 4633933..4634106 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag E4V11_RS22700 Protein ID WP_000398619.1
Coordinates 4634134..4634430 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4V11_RS22685 4630603..4632060 + 1458 WP_066009710.1 dipeptide/tripeptide permease DtpC -
E4V11_RS22690 4632297..4633814 + 1518 WP_066009713.1 lysine--tRNA ligase -
E4V11_RS22695 4633933..4634106 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
E4V11_RS22700 4634134..4634430 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
E4V11_RS22705 4634657..4634929 - 273 WP_000405651.1 N-acetyltransferase -
E4V11_RS22710 4634941..4635171 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
E4V11_RS22715 4635352..4636983 + 1632 WP_066009715.1 sensor histidine kinase -
E4V11_RS22720 4636980..4637699 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4588659..4634430 45771


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T288371 WP_001173343.1 NZ_LR217818:c4634106-4633933 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT288371 WP_000398619.1 NZ_LR217818:c4634430-4634134 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References