288368

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4296648..4297224 Replicon chromosome
Accession NZ_LR217818
Organism Escherichia coli strain NCTC86

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag E4V11_RS20900 Protein ID WP_001297643.1
Coordinates 4296937..4297224 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag E4V11_RS20895 Protein ID WP_000063156.1
Coordinates 4296648..4296950 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4V11_RS20880 4293244..4295394 + 2151 WP_066009113.1 pyruvate/proton symporter BtsT -
E4V11_RS20885 4295444..4295647 + 204 WP_000467860.1 YbdD/YjiX family protein -
E4V11_RS20890 4295658..4296614 + 957 WP_001297640.1 GTPase -
E4V11_RS20895 4296648..4296950 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
E4V11_RS20900 4296937..4297224 - 288 WP_001297643.1 BrnT family toxin Toxin
E4V11_RS20905 4297423..4298119 - 697 Protein_4037 IS1 family transposase -
E4V11_RS20910 4298434..4299666 + 1233 WP_001137032.1 multidrug efflux MFS transporter MdtM -
E4V11_RS20915 4299707..4300987 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4297423..4297632 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T288368 WP_001297643.1 NZ_LR217818:c4297224-4296937 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT288368 WP_000063156.1 NZ_LR217818:c4296950-4296648 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References