Detailed information of TA system TA288355

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2118110..2118954
• Replicon: chromosome
• Accession: NZ_LR217818
• Organism: Escherichia coli strain NCTC86

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: E4V11_RS10375
• Protein ID: WP_000854686.1
• Coordinates: 2118571..2118954 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: E4V11_RS10370
• Protein ID: WP_001285602.1
• Coordinates: 2118110..2118490 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4V11_RS10330	2114358..2114813	+	456	WP_000581502.1	hypothetical protein	-
E4V11_RS10335	2114892..2115125	+	234	WP_001119727.1	DUF905 family protein	-
E4V11_RS10345	2115226..2116044	+	819	WP_068879456.1	DUF945 domain-containing protein	-
E4V11_RS10350	2116136..2116621	+	486	WP_000214307.1	antirestriction protein	-
E4V11_RS10355	2116637..2117113	+	477	WP_021543704.1	RadC family protein	-
E4V11_RS10360	2117176..2117397	+	222	WP_000692298.1	DUF987 domain-containing protein	-
E4V11_RS10365	2117416..2118099	+	684	WP_000086768.1	hypothetical protein	-
E4V11_RS10370	2118110..2118490	+	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E4V11_RS10375	2118571..2118954	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
E4V11_RS10380	2118951..2119439	+	489	WP_001054233.1	hypothetical protein	-
E4V11_RS10385	2119456..2119653	+	198	WP_000839256.1	DUF957 domain-containing protein	-
E4V11_RS10390	2119738..2120580	+	843	WP_068879455.1	DUF4942 domain-containing protein	-
E4V11_RS10400	2121072..2121290	-	219	WP_001040187.1	translation initiation factor IF-1	-
E4V11_RS10405	2121575..2122279	-	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T288355 WP_000854686.1 NZ_LR217818:2118571-2118954 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT288355 WP_001285602.1 NZ_LR217818:2118110-2118490 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp