288354

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 2099613..2101333 Replicon chromosome
Accession NZ_LR217818
Organism Escherichia coli strain NCTC86

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag E4V11_RS10285 Protein ID WP_000312833.1
Coordinates 2100677..2101333 (-) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag E4V11_RS10280 Protein ID WP_000634203.1
Coordinates 2099613..2100680 (-) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4V11_RS10260 2095259..2095438 - 180 Protein_1970 IS3 family transposase -
E4V11_RS10270 2095783..2096499 - 717 WP_001122107.1 M48 family metallopeptidase -
E4V11_RS10275 2096499..2099594 - 3096 WP_000108735.1 HsdR family type I site-specific deoxyribonuclease -
E4V11_RS10280 2099613..2100680 - 1068 WP_000634203.1 macro domain-containing protein Antitoxin
E4V11_RS10285 2100677..2101333 - 657 WP_000312833.1 DUF4433 domain-containing protein Toxin
E4V11_RS10290 2101346..2102679 - 1334 Protein_1975 restriction endonuclease subunit S -
E4V11_RS10295 2102679..2105117 - 2439 WP_000985465.1 type I restriction-modification system subunit M -
E4V11_RS10300 2105157..2105396 - 240 WP_001431807.1 hypothetical protein -
E4V11_RS24970 2105678..2105932 + 255 WP_154670580.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - iroN / iroE / iroE / iroD / iroC / iroC / iroC / aggD / aafC / agg3C 2068750..2107339 38589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T288354 WP_000312833.1 NZ_LR217818:c2101333-2100677 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT288354 WP_000634203.1 NZ_LR217818:c2100680-2099613 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References