Detailed information of TA system TA288330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 968176..969413
• Replicon: chromosome
• Accession: NZ_LR216050
• Organism: Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: E0F32_RS05290
• Protein ID: WP_000405365.1
• Coordinates: 968652..969413 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: E0F32_RS05285
• Protein ID: WP_000579599.1
• Coordinates: 968176..968652 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0F32_RS05265	963249..964103	+	855	WP_000900020.1	ADP-ribosylglycohydrolase family protein	-
E0F32_RS05270	964215..965099	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
E0F32_RS05275	965262..967001	+	1740	WP_000423466.1	glycoside hydrolase family 13 protein	-
E0F32_RS05280	967276..967731	+	456	WP_000500465.1	hypothetical protein	-
E0F32_RS05285	968176..968652	+	477	WP_000579599.1	helix-turn-helix domain-containing protein	Antitoxin
E0F32_RS05290	968652..969413	+	762	WP_000405365.1	zeta toxin family protein	Toxin
E0F32_RS05295	969441..970597	+	1157	Protein_990	hypothetical protein	-
E0F32_RS05305	971416..971775	+	360	WP_000003745.1	hypothetical protein	-
E0F32_RS05310	971785..972150	+	366	WP_000263051.1	MobC family plasmid mobilization relaxosome protein	-
E0F32_RS05315	972137..974002	+	1866	WP_000261795.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(M)	-	881886..970880	88994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29060.05 Da
• Isoelectric Point: 6.8511

>T288330 WP_000405365.1 NZ_LR216050:968652-969413 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18251.60 Da
• Isoelectric Point: 4.3571

>AT288330 WP_000579599.1 NZ_LR216050:968176-968652 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQDDFSRIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp