Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1666794..1667392 Replicon chromosome
Accession NC_020210
Organism Geobacillus sp. GHH01

Toxin (Protein)


Gene name mazF Uniprot ID G8N5V4
Locus tag GHH_RS08210 Protein ID WP_011231143.1
Coordinates 1667063..1667392 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G8N5V3
Locus tag GHH_RS08205 Protein ID WP_011231142.1
Coordinates 1666794..1667063 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHH_RS08165 1662543..1662830 + 288 WP_041470695.1 transporter suffix domain-containing protein -
GHH_RS08170 1662848..1663645 + 798 WP_015374774.1 SGNH/GDSL hydrolase family protein -
GHH_RS08175 1663642..1664556 + 915 WP_015374775.1 ABC transporter ATP-binding protein -
GHH_RS08180 1664549..1665529 + 981 WP_015374776.1 ABC transporter permease -
GHH_RS08185 1665598..1666107 - 510 WP_015374777.1 hypothetical protein -
GHH_RS08205 1666794..1667063 + 270 WP_011231142.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GHH_RS08210 1667063..1667392 + 330 WP_011231143.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GHH_RS18180 1667424..1667663 - 240 WP_020753918.1 hypothetical protein -
GHH_RS08215 1667849..1668682 + 834 WP_015374778.1 pirin family protein -
GHH_RS08220 1668778..1669527 + 750 WP_015374779.1 oxygen-insensitive NADPH nitroreductase -
GHH_RS08225 1669601..1670344 + 744 WP_015374780.1 NAD-dependent protein deacylase -
GHH_RS08230 1670525..1671703 + 1179 WP_015374781.1 acyl-CoA dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-107)

Antitoxin

Antitoxin PemI-like

(12-87)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12071.86 Da        Isoelectric Point: 7.1331

>T28833 WP_011231143.1 NC_020210:1667063-1667392 [Geobacillus sp. GHH01]
MQAPDRGDLVYVNFNPQAGHEQAGKRPGIVLSPKRFNQLTGFAVLCPITRQQKGYPFEVELPSGLAVEGVILTDQVKSLD
WRARQLQIVGRAPNEVVSDCLDLIHTFLS

Download         Length: 330 bp

>T28833 NC_020210:1667063-1667392 [Geobacillus sp. GHH01]
ATGCAAGCGCCGGATCGTGGGGATCTTGTTTACGTCAATTTCAATCCACAAGCAGGGCATGAGCAGGCAGGAAAAAGGCC
CGGCATCGTTCTATCACCAAAGCGGTTCAATCAATTGACAGGGTTTGCGGTGCTTTGCCCGATCACCCGGCAACAAAAAG
GATATCCATTTGAAGTGGAATTGCCATCAGGCTTGGCCGTTGAAGGCGTGATTTTAACCGATCAGGTCAAAAGTTTAGAT
TGGCGCGCCCGACAGCTTCAAATAGTAGGACGGGCGCCCAATGAAGTTGTTTCGGATTGCTTGGATCTGATTCATACTTT
TCTTTCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9992.67 Da        Isoelectric Point: 7.2050

>AT28833 WP_011231142.1 NC_020210:1666794-1667063 [Geobacillus sp. GHH01]
MELNKQKGVSIMTITVQKWGNSLAVRIPSVIAERLALHQGSEVEMIVENQAIKLIPKKKKPTLEELLAKITPENRHAEID
FGTEGNELF

Download         Length: 270 bp

>AT28833 NC_020210:1666794-1667063 [Geobacillus sp. GHH01]
ATGGAATTAAACAAACAGAAAGGAGTGTCAATCATGACAATAACCGTTCAAAAATGGGGAAACAGCCTTGCGGTTCGTAT
CCCAAGCGTGATTGCTGAACGTTTAGCGCTTCATCAAGGATCAGAAGTGGAGATGATCGTTGAGAACCAAGCGATCAAGT
TGATCCCGAAAAAGAAGAAGCCGACATTGGAGGAACTTTTGGCTAAAATCACGCCGGAAAATCGCCATGCTGAAATTGAT
TTTGGAACAGAAGGGAATGAATTGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9EUB7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9HWH2

References