Detailed information of TA system TA288329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 938937..940180
• Replicon: chromosome
• Accession: NZ_LR216050
• Organism: Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: E0F32_RS05140
• Protein ID: WP_024477678.1
• Coordinates: 939413..940180 (+)
• Length: 256 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: E0F32_RS05135
• Protein ID: WP_000579597.1
• Coordinates: 938937..939413 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0F32_RS05095	935191..935475	+	285	WP_000969635.1	hypothetical protein	-
E0F32_RS05100	935489..935785	+	297	WP_024478354.1	hypothetical protein	-
E0F32_RS05105	935799..935996	-	198	WP_002876112.1	hypothetical protein	-
E0F32_RS05110	936156..936797	+	642	WP_000933087.1	hypothetical protein	-
E0F32_RS05115	936807..937892	+	1086	WP_000401910.1	DUF3991 domain-containing protein	-
E0F32_RS05120	937943..938176	+	234	WP_000152425.1	hypothetical protein	-
E0F32_RS05125	938173..938556	+	384	WP_002933294.1	hypothetical protein	-
E0F32_RS05130	938594..938866	+	273	WP_024477679.1	hypothetical protein	-
E0F32_RS05135	938937..939413	+	477	WP_000579597.1	helix-turn-helix domain-containing protein	Antitoxin
E0F32_RS05140	939413..940180	+	768	WP_024477678.1	zeta toxin family protein	Toxin
E0F32_RS05145	940215..941834	+	1620	WP_078133416.1	DUF4297 domain-containing protein	-
E0F32_RS05150	941821..942360	+	540	WP_024477676.1	hypothetical protein	-
E0F32_RS05155	942350..944989	+	2640	WP_078133417.1	SMC family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(M)	-	881886..970880	88994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 256 a.a.
• Molecular weight: 29310.39 Da
• Isoelectric Point: 7.4386

>T288329 WP_024477678.1 NZ_LR216050:939413-940180 [Streptococcus pneumoniae]
MGIQDYTDSEFKQALERNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFARKMVESLVTELSHLGYNLLIEGTLRTIDVPKKTAQLLKSRGYEVQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEKLAIFERIQIYQRDRSCVYDSKENTTSAAEVLQECLFGKWSKVEEEML
KVGEEILETLCNRTE

Nucleotide

• Length: 768 bp

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18235.60 Da
• Isoelectric Point: 4.3571

>AT288329 WP_000579597.1 NZ_LR216050:938937-939413 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQDDFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp