Detailed information of TA system TA028828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 827430..828044
• Replicon: chromosome
• Accession: NC_020209
• Organism: Pseudomonas poae RE*1-1-14

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L7HGV1
• Locus tag: H045_RS24310
• Protein ID: WP_003233882.1
• Coordinates: 827862..828044 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L7HIG7
• Locus tag: H045_RS03780
• Protein ID: WP_003233880.1
• Coordinates: 827430..827840 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H045_RS03745	822782..823705	+	924	WP_208597733.1	LysR family transcriptional regulator	-
H045_RS03750	823724..824134	+	411	WP_003233868.1	fosfomycin resistance glutathione transferase	-
H045_RS03755	824187..824828	+	642	WP_015371471.1	LysE family translocator	-
H045_RS03760	824948..825475	+	528	WP_015371472.1	DUF4142 domain-containing protein	-
H045_RS03765	825530..825955	-	426	WP_015371473.1	low affinity iron permease family protein	-
H045_RS03770	826131..826913	+	783	WP_015371474.1	GNAT family N-acetyltransferase	-
H045_RS03775	826914..827414	-	501	WP_015371475.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
H045_RS03780	827430..827840	-	411	WP_003233880.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H045_RS24310	827862..828044	-	183	WP_003233882.1	type II toxin-antitoxin system HicA family toxin	Toxin
H045_RS03785	828222..828527	-	306	WP_003233884.1	hypothetical protein	-
H045_RS03790	828549..829073	-	525	WP_003233886.1	DUF3833 domain-containing protein	-
H045_RS03800	829440..830324	-	885	WP_003233888.1	alpha/beta hydrolase	-
H045_RS03805	830407..831099	-	693	WP_015371478.1	pseudouridine synthase	-
H045_RS03810	831089..831301	-	213	WP_032870483.1	cysteine-rich CWC family protein	-
H045_RS03815	831364..832986	-	1623	WP_015371480.1	methyl-accepting chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6820.01 Da
• Isoelectric Point: 10.5955

>T28828 WP_003233882.1 NC_020209:c828044-827862 [Pseudomonas poae RE*1-1-14]
VDSRYLIGHIVADGWYLVRIRGSHHHYKHPTKPGLVTIPHPKKDLLDKTAKSILKQALLS

Nucleotide

• Length: 183 bp

>T28828 NC_020209:c828044-827862 [Pseudomonas poae RE*1-1-14]
GTGGATAGTCGGTATTTGATAGGTCATATCGTTGCGGATGGCTGGTACCTGGTGCGTATCAGAGGCAGTCATCACCACTA
CAAACACCCGACCAAGCCCGGACTGGTGACGATTCCTCATCCAAAAAAGGATCTGTTGGACAAGACTGCCAAAAGCATTT
TGAAACAGGCTCTGCTGAGTTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14674.55 Da
• Isoelectric Point: 4.8835

>AT28828 WP_003233880.1 NC_020209:c827840-827430 [Pseudomonas poae RE*1-1-14]
MLYPIAISTGDKDHAWGVEVPDIPGCHSAGDDLDDAMAMAREAIEGHFEILAEDGSPIPGAQKVTVHAANPQYAGCTWAV
VDIDVTRYLGKAQKLNITLPGYLLNRIDDYVLHHPEEKSRSGFLASAALKVLQNGH

Nucleotide

• Length: 411 bp

>AT28828 NC_020209:c827840-827430 [Pseudomonas poae RE*1-1-14]
ATGCTGTACCCCATCGCAATTTCAACCGGTGACAAAGACCACGCCTGGGGCGTGGAAGTCCCGGATATTCCTGGCTGCCA
TTCGGCCGGCGACGACCTGGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGACACTTCGAAATCCTTGCCGAAG
ACGGCTCGCCGATTCCCGGCGCGCAAAAAGTCACGGTGCATGCCGCCAACCCCCAATACGCCGGCTGTACCTGGGCAGTG
GTGGACATCGATGTCACCCGCTATCTGGGCAAGGCGCAAAAACTCAACATCACCTTGCCTGGCTATCTGCTTAATCGCAT
TGATGACTATGTGCTACACCACCCGGAAGAAAAAAGCCGTTCAGGTTTTCTGGCATCGGCAGCACTCAAGGTACTGCAGA
ACGGCCACTGA

Structures

Toxin

• AlphaFold DB: L7HGV1

Antitoxin

• AlphaFold DB: L7HIG7