Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 477450..478021 Replicon chromosome
Accession NC_020207
Organism Enterococcus faecium NRRL B-2354

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag M7W_RS02255 Protein ID WP_002286801.1
Coordinates 477680..478021 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag M7W_RS02250 Protein ID WP_002323011.1
Coordinates 477450..477680 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M7W_RS02225 472570..473901 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
M7W_RS02230 473923..474549 + 627 WP_002286816.1 cysteine hydrolase -
M7W_RS02235 474732..475313 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
M7W_RS02240 476045..476620 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
M7W_RS02245 476825..477163 - 339 WP_002286804.1 hypothetical protein -
M7W_RS02250 477450..477680 + 231 WP_002323011.1 hypothetical protein Antitoxin
M7W_RS02255 477680..478021 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M7W_RS02260 478871..479056 + 186 WP_002304207.1 hypothetical protein -
M7W_RS02270 479514..480676 + 1163 WP_123836059.1 IS3 family transposase -
M7W_RS02275 480930..481124 + 195 WP_002295146.1 hypothetical protein -
M7W_RS14070 481315..481564 + 250 Protein_452 transposase -
M7W_RS02285 481808..482638 - 831 WP_002286789.1 manganese catalase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T28826 WP_002286801.1 NC_020207:477680-478021 [Enterococcus faecium ATCC 8459 = NRRL B-2354]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T28826 NC_020207:477680-478021 [Enterococcus faecium ATCC 8459 = NRRL B-2354]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT28826 WP_002323011.1 NC_020207:477450-477680 [Enterococcus faecium ATCC 8459 = NRRL B-2354]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT28826 NC_020207:477450-477680 [Enterococcus faecium ATCC 8459 = NRRL B-2354]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References