Detailed information of TA system TA028825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/Txe-RelB
• Location: 188542..189071
• Replicon: chromosome
• Accession: NC_020207
• Organism: Enterococcus faecium NRRL B-2354

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: M7W_RS00930
• Protein ID: WP_010738607.1
• Coordinates: 188805..189071 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: C9B8W8
• Locus tag: M7W_RS00925
• Protein ID: WP_002294199.1
• Coordinates: 188542..188805 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M7W_RS00915	185917..187740	+	1824	WP_002294202.1	APC family permease	-
M7W_RS00920	187882..188067	-	186	WP_002294201.1	hypothetical protein	-
M7W_RS00925	188542..188805	+	264	WP_002294199.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
M7W_RS00930	188805..189071	+	267	WP_010738607.1	Txe/YoeB family addiction module toxin	Toxin
M7W_RS00935	189272..189760	+	489	WP_002294196.1	mannosyl-glycoprotein endo-beta-N-acetylglucosamidase	-
M7W_RS00940	190302..190973	-	672	WP_002319469.1	ABC transporter permease	-
M7W_RS00945	190970..191887	-	918	WP_002294193.1	osmoprotectant ABC transporter substrate-binding protein	-
M7W_RS00950	191884..192525	-	642	WP_002293405.1	ABC transporter permease	-
M7W_RS00955	192529..193704	-	1176	WP_002294192.1	betaine/proline/choline family ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (22-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10647.05 Da
• Isoelectric Point: 9.6068

>T28825 WP_010738607.1 NC_020207:188805-189071 [Enterococcus faecium ATCC 8459 = NRRL B-2354]
MSNYTVAIKNSAKVDLRKIEQSNLKKQFEEVIQTLKEDPYMPTQSFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEI
YSAWTHYE

Nucleotide

• Length: 267 bp

>T28825 NC_020207:188805-189071 [Enterococcus faecium ATCC 8459 = NRRL B-2354]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCGAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGTTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10016.03 Da
• Isoelectric Point: 4.1207

>AT28825 WP_002294199.1 NC_020207:188542-188805 [Enterococcus faecium ATCC 8459 = NRRL B-2354]
MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIGLEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNID
DIDWEAL

Nucleotide

• Length: 264 bp

>AT28825 NC_020207:188542-188805 [Enterococcus faecium ATCC 8459 = NRRL B-2354]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A828ZU84