Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 37642..38779 Replicon plasmid 4
Accession NZ_LR135491
Organism Enterococcus faecium isolate E8414

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag EQB57_RS18030 Protein ID WP_077495093.1
Coordinates 37642..38505 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID A0A7U7LLF6
Locus tag EQB57_RS18035 Protein ID WP_031644662.1
Coordinates 38507..38779 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB57_RS18000 33095..33712 - 618 WP_099124473.1 FtsX-like permease family protein -
EQB57_RS18005 33716..34396 - 681 WP_060854029.1 IS6-like element IS1216 family transposase -
EQB57_RS18010 34543..34626 + 84 WP_001860874.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
EQB57_RS18015 34751..35488 + 738 WP_002321849.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
EQB57_RS18020 35792..36196 + 405 WP_002287522.1 IS200/IS605-like element ISEfa4 family transposase -
EQB57_RS18025 36213..37361 + 1149 WP_002287525.1 IS200/IS605 family element transposase accessory protein TnpB -
EQB57_RS18030 37642..38505 - 864 WP_077495093.1 zeta toxin family protein Toxin
EQB57_RS18035 38507..38779 - 273 WP_031644662.1 antitoxin Antitoxin
EQB57_RS18040 38797..39006 - 210 WP_000527317.1 peptide-binding protein -
EQB57_RS18045 39083..39979 - 897 WP_099119832.1 ParA family protein -
EQB57_RS18050 40082..41569 - 1488 WP_077495095.1 type IA DNA topoisomerase -
EQB57_RS18055 41760..42699 - 940 WP_127822056.1 IS1380-like element ISSsu5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(6)-Ia / VanHAX / erm(B) - 1..42699 42699
- inside IScluster/Tn erm(B) - 33716..42692 8976


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(26-216)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32489.07 Da        Isoelectric Point: 8.2810

>T288234 WP_077495093.1 NZ_LR135491:c38505-37642 [Enterococcus faecium]
MANIVNFTDKQFENRLRDNIEELVQGKKAVESPTAFLLGGQPGSGKTSLRSAIYEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKSYETKMYVMAVPKIESYLGTIERYE
TMFSDEPLTARATPKQAHDIVVKNLPINLDKLHKTGLFSDIRLYNRSGVKLYSSSETPSISPKETLERELNRKLSGKEIQ
PTLERVEQKMIQNKHQEAPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10735.36 Da        Isoelectric Point: 8.3744

>AT288234 WP_031644662.1 NZ_LR135491:c38779-38507 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKDNSQLLEVNLLNQLKLAKRVNLFDYSLEELKAVHEYWRSMNRYS
KQVLKKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U7LLF6

References