Detailed information of TA system TA288234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 37642..38779
• Replicon: plasmid 4
• Accession: NZ_LR135491
• Organism: Enterococcus faecium isolate E8414

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: EQB57_RS18030
• Protein ID: WP_077495093.1
• Coordinates: 37642..38505 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: A0A7U7LLF6
• Locus tag: EQB57_RS18035
• Protein ID: WP_031644662.1
• Coordinates: 38507..38779 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQB57_RS18000	33095..33712	-	618	WP_099124473.1	FtsX-like permease family protein	-
EQB57_RS18005	33716..34396	-	681	WP_060854029.1	IS6-like element IS1216 family transposase	-
EQB57_RS18010	34543..34626	+	84	WP_001860874.1	23S rRNA methyltransferase attenuator leader peptide ErmL	-
EQB57_RS18015	34751..35488	+	738	WP_002321849.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
EQB57_RS18020	35792..36196	+	405	WP_002287522.1	IS200/IS605-like element ISEfa4 family transposase	-
EQB57_RS18025	36213..37361	+	1149	WP_002287525.1	IS200/IS605 family element transposase accessory protein TnpB	-
EQB57_RS18030	37642..38505	-	864	WP_077495093.1	zeta toxin family protein	Toxin
EQB57_RS18035	38507..38779	-	273	WP_031644662.1	antitoxin	Antitoxin
EQB57_RS18040	38797..39006	-	210	WP_000527317.1	peptide-binding protein	-
EQB57_RS18045	39083..39979	-	897	WP_099119832.1	ParA family protein	-
EQB57_RS18050	40082..41569	-	1488	WP_077495095.1	type IA DNA topoisomerase	-
EQB57_RS18055	41760..42699	-	940	WP_127822056.1	IS1380-like element ISSsu5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ant(6)-Ia / VanHAX / erm(B)	-	1..42699	42699
	-	inside	IScluster/Tn	erm(B)	-	33716..42692	8976

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (26-216)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32489.07 Da
• Isoelectric Point: 8.2810

>T288234 WP_077495093.1 NZ_LR135491:c38505-37642 [Enterococcus faecium]
MANIVNFTDKQFENRLRDNIEELVQGKKAVESPTAFLLGGQPGSGKTSLRSAIYEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKSYETKMYVMAVPKIESYLGTIERYE
TMFSDEPLTARATPKQAHDIVVKNLPINLDKLHKTGLFSDIRLYNRSGVKLYSSSETPSISPKETLERELNRKLSGKEIQ
PTLERVEQKMIQNKHQEAPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10735.36 Da
• Isoelectric Point: 8.3744

>AT288234 WP_031644662.1 NZ_LR135491:c38779-38507 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKDNSQLLEVNLLNQLKLAKRVNLFDYSLEELKAVHEYWRSMNRYS
KQVLKKEKVA

Nucleotide

• Length: 273 bp

Structures

Antitoxin

• AlphaFold DB: A0A7U7LLF6