288233

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 2790..3898 Replicon plasmid 4
Accession NZ_LR135491
Organism Enterococcus faecium isolate E8414

Toxin (Protein)


Gene name MNTss Uniprot ID R3JIN1
Locus tag EQB57_RS17830 Protein ID WP_000233000.1
Coordinates 2790..3659 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag EQB57_RS17835 Protein ID WP_000205227.1
Coordinates 3674..3898 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB57_RS17810 1..380 - 380 WP_197724957.1 transposase -
EQB57_RS17815 608..1135 - 528 WP_002294507.1 adenine phosphoribosyltransferase -
EQB57_RS17820 1179..2042 - 864 WP_002294505.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB57_RS17825 2075..2809 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB57_RS17830 2790..3659 - 870 WP_000233000.1 nucleotidyltransferase domain-containing protein Toxin
EQB57_RS17835 3674..3898 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
EQB57_RS17840 4041..5603 - 1563 WP_000136908.1 recombinase family protein -
EQB57_RS17845 5605..6021 - 417 WP_000323438.1 recombinase -
EQB57_RS17850 6022..6318 - 297 WP_002360685.1 hypothetical protein -
EQB57_RS17855 6830..7507 - 678 Protein_9 DNA topoisomerase -
EQB57_RS17860 7523..8125 - 603 WP_001062586.1 recombinase family protein -
EQB57_RS18450 8139..8309 - 171 WP_000713595.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(6)-Ia / VanHAX / erm(B) - 1..42699 42699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32926.57 Da        Isoelectric Point: 4.8781

>T288233 WP_000233000.1 NZ_LR135491:c3659-2790 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT288233 WP_000205227.1 NZ_LR135491:c3898-3674 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3JIN1

Antitoxin

Source ID Structure

References