Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 499346..499917 Replicon chromosome
Accession NZ_LR135488
Organism Enterococcus faecium isolate E8414

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag EQB57_RS02560 Protein ID WP_002286801.1
Coordinates 499576..499917 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag EQB57_RS02555 Protein ID WP_002323011.1
Coordinates 499346..499576 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB57_RS02505 494479..495810 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
EQB57_RS02510 495832..496458 + 627 WP_002286816.1 cysteine hydrolase -
EQB57_RS02515 496641..497222 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
EQB57_RS02535 497954..498301 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
EQB57_RS02540 498277..498516 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
EQB57_RS02545 498721..499059 - 339 WP_002286804.1 hypothetical protein -
EQB57_RS02555 499346..499576 + 231 WP_002323011.1 hypothetical protein Antitoxin
EQB57_RS02560 499576..499917 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EQB57_RS02565 500767..500952 + 186 WP_002304207.1 hypothetical protein -
EQB57_RS02570 501457..501651 + 195 WP_002297028.1 hypothetical protein -
EQB57_RS02575 501809..502091 + 283 Protein_476 transposase -
EQB57_RS02585 502335..503165 - 831 WP_002286789.1 manganese catalase family protein -
EQB57_RS02590 503373..504707 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T288231 WP_002286801.1 NZ_LR135488:499576-499917 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT288231 WP_002323011.1 NZ_LR135488:499346-499576 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References