Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 149703..150811 Replicon plasmid 2
Accession NZ_LR135483
Organism Enterococcus faecium isolate E4456

Toxin (Protein)


Gene name MNTss Uniprot ID R3JIN1
Locus tag EQB55_RS14455 Protein ID WP_000233000.1
Coordinates 149703..150572 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag EQB55_RS14460 Protein ID WP_000205227.1
Coordinates 150587..150811 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB55_RS14430 144812..145783 - 972 Protein_158 type IA DNA topoisomerase -
EQB55_RS14435 145974..147293 - 1320 WP_002338419.1 IS1380-like element ISSsu5 family transposase -
EQB55_RS14440 147521..148048 - 528 WP_002294507.1 adenine phosphoribosyltransferase -
EQB55_RS14445 148092..148955 - 864 WP_002294505.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB55_RS14450 148988..149722 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB55_RS14455 149703..150572 - 870 WP_000233000.1 nucleotidyltransferase domain-containing protein Toxin
EQB55_RS14460 150587..150811 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
EQB55_RS14470 151249..152531 + 1283 Protein_165 ISL3 family transposase -
EQB55_RS14480 152840..153643 - 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
EQB55_RS14485 153697..155181 - 1485 WP_002294513.1 ABC-F type ribosomal protection protein Lsa(E) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32926.57 Da        Isoelectric Point: 4.8781

>T288228 WP_000233000.1 NZ_LR135483:c150572-149703 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT288228 WP_000205227.1 NZ_LR135483:c150811-150587 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3JIN1

Antitoxin

Source ID Structure

References