Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 3691..4828 Replicon plasmid 3
Accession NZ_LR135477
Organism Enterococcus faecium isolate E8423

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag EQB52_RS15325 Protein ID WP_001284311.1
Coordinates 3691..4554 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag EQB52_RS15330 Protein ID WP_000301765.1
Coordinates 4556..4828 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB52_RS15305 1..1041 + 1041 WP_000997689.1 replication initiator protein A -
EQB52_RS15310 1657..2337 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EQB52_RS15315 2483..2566 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
EQB52_RS15320 2691..3428 + 738 WP_001038792.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
EQB52_RS15325 3691..4554 - 864 WP_001284311.1 toxin zeta Toxin
EQB52_RS15330 4556..4828 - 273 WP_000301765.1 antitoxin Antitoxin
EQB52_RS15335 4845..5054 - 210 WP_000527318.1 peptide-binding protein -
EQB52_RS15340 5219..5899 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EQB52_RS15345 5974..6786 - 813 Protein_8 peptidase -
EQB52_RS15350 6839..7510 - 672 WP_127824112.1 class A sortase -
EQB52_RS15355 7596..7835 - 240 WP_002296346.1 hypothetical protein -
EQB52_RS15360 7902..8141 - 240 WP_002321270.1 hypothetical protein -
EQB52_RS15365 8255..8596 - 342 WP_060812635.1 DUF5406 domain-containing protein -
EQB52_RS15370 8583..8912 - 330 WP_002296227.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / VanHAX - 1..44000 44000
- inside IScluster/Tn erm(B) - 1657..5905 4248


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T288224 WP_001284311.1 NZ_LR135477:c4554-3691 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT288224 WP_000301765.1 NZ_LR135477:c4828-4556 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References