Detailed information of TA system TA028822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4030854..4031875
• Replicon: chromosome
• Accession: NC_020181
• Organism: Enterobacter aerogenes EA1509E

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A0G9UXE3
• Locus tag: EAEROS_RS19755
• Protein ID: WP_032706547.1
• Coordinates: 4031300..4031875 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A0G9R618
• Locus tag: EAEROS_RS19750
• Protein ID: WP_015369110.1
• Coordinates: 4030854..4031300 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAEROS_RS19725	4026375..4027154	+	780	WP_015369105.1	MBL fold metallo-hydrolase	-
EAEROS_RS19730	4027176..4028129	+	954	WP_032706577.1	helix-turn-helix domain-containing protein	-
EAEROS_RS19735	4028126..4029175	-	1050	WP_015369107.1	NAD(P)-dependent alcohol dehydrogenase	-
EAEROS_RS19740	4029333..4029635	-	303	WP_015369108.1	antibiotic biosynthesis monooxygenase	-
EAEROS_RS19745	4029636..4030643	-	1008	WP_015369109.1	zinc-binding alcohol dehydrogenase family protein	-
EAEROS_RS19750	4030854..4031300	+	447	WP_015369110.1	helix-turn-helix domain-containing protein	Antitoxin
EAEROS_RS19755	4031300..4031875	+	576	WP_032706547.1	PIN domain-containing protein	Toxin
EAEROS_RS19765	4032180..4033376	+	1197	WP_015369112.1	hypothetical protein	-
EAEROS_RS19770	4033553..4034590	-	1038	WP_041165586.1	virulence RhuM family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• SinI-like, DNA-binding domain (79-126)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21688.29 Da
• Isoelectric Point: 6.2900

>T28822 WP_032706547.1 NC_020181:4031300-4031875 [Klebsiella aerogenes EA1509E]
MRHSPYPVILDACVLYPARLRDLLMHLGIAGLYQPKWSRFIHDEWCRNLLINRPDIAPEALRRTVDLMNAALPDANVTGF
AGLINGLVLPDPDDRHVLAAAIRAKAEIIVTLNHKDFPAESLLPFEIETLHPDVFISDLFDLNHALALEAVRRQRQSLKH
PPMTVDEFLEMLLKQGLPMTVKELANYQYAI

Nucleotide

• Length: 576 bp

>T28822 NC_020181:4031300-4031875 [Klebsiella aerogenes EA1509E]
ATGCGACATTCACCTTATCCGGTGATTTTAGATGCTTGTGTATTATATCCAGCACGGCTCAGGGATTTATTAATGCACTT
AGGGATCGCTGGACTTTATCAACCAAAATGGAGCCGCTTCATTCACGATGAATGGTGCAGGAATCTTCTTATAAATCGGC
CTGATATAGCACCTGAAGCGCTGCGTCGTACCGTTGATCTCATGAATGCAGCTTTACCGGATGCTAATGTAACGGGTTTT
GCAGGATTAATTAACGGACTAGTTTTACCCGACCCTGACGATCGGCATGTCCTTGCTGCGGCAATCAGAGCGAAGGCTGA
AATTATCGTTACGTTAAATCATAAAGACTTTCCAGCAGAATCATTGCTTCCATTTGAAATCGAAACACTTCATCCTGATG
TATTCATATCGGACCTGTTTGACCTTAATCATGCTCTGGCGTTGGAGGCGGTGAGAAGGCAGCGGCAGTCTCTCAAGCAT
CCTCCAATGACCGTCGATGAGTTCCTGGAGATGCTGCTAAAGCAGGGCTTGCCGATGACGGTAAAAGAGTTAGCCAATTA
CCAGTACGCCATATAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16699.26 Da
• Isoelectric Point: 6.2257

>AT28822 WP_015369110.1 NC_020181:4030854-4031300 [Klebsiella aerogenes EA1509E]
MTKLNLPDPLESELAQRGQRELAAYLSTHLETQRIAIVGEDDKTHTIELPTSALTMLMEILGELACGNAVQIVPVHAELT
TQEAANILNVSRPHMVKLLEARKLPFHKTGRHRRVLFADLMEYKKRREQESLDAMQALADQAQDLGMY

Nucleotide

• Length: 447 bp

>AT28822 NC_020181:4030854-4031300 [Klebsiella aerogenes EA1509E]
ATGACTAAACTGAACCTTCCGGACCCGCTGGAGAGTGAACTTGCCCAGCGCGGGCAGCGTGAGCTGGCGGCATACCTTTC
TACTCATCTTGAAACGCAGCGTATTGCGATTGTGGGTGAAGATGACAAAACTCATACCATTGAGTTGCCAACCAGCGCTC
TGACTATGCTGATGGAGATCCTTGGGGAATTGGCTTGTGGCAACGCGGTGCAGATTGTTCCTGTTCATGCTGAACTCACC
ACCCAGGAAGCAGCGAATATACTCAATGTTTCTCGTCCGCATATGGTTAAACTGCTGGAAGCGAGGAAGCTTCCATTTCA
TAAAACTGGACGCCATCGCCGGGTTCTGTTTGCCGATCTGATGGAGTATAAAAAACGTAGGGAACAGGAAAGCCTGGACG
CGATGCAAGCGCTGGCGGATCAGGCGCAGGACTTAGGGATGTATTAA

Structures

Toxin

• AlphaFold DB: A0A0G9UXE3

Antitoxin

• AlphaFold DB: A0A0G9R618