Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 20239..21347 Replicon plasmid 4
Accession NZ_LR135422
Organism Enterococcus faecium isolate E8440

Toxin (Protein)


Gene name MNTss Uniprot ID -
Locus tag FQ475_RS16155 Protein ID WP_060763469.1
Coordinates 20239..21108 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag FQ475_RS16160 Protein ID WP_000205227.1
Coordinates 21123..21347 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FQ475_RS16130 16309..16881 - 573 WP_001079938.1 HTH domain-containing protein -
FQ475_RS16135 17157..17951 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
FQ475_RS16140 18044..18586 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
FQ475_RS16145 18583..19491 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
FQ475_RS16150 19524..20258 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
FQ475_RS16155 20239..21108 - 870 WP_060763469.1 nucleotidyltransferase domain-containing protein Toxin
FQ475_RS16160 21123..21347 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
FQ475_RS16165 21490..23052 - 1563 WP_000136908.1 recombinase family protein -
FQ475_RS16170 23054..23470 - 417 WP_000323438.1 recombinase -
FQ475_RS16175 23471..23968 - 498 WP_002327635.1 DNA recombinase -
FQ475_RS16180 24279..24956 - 678 Protein_30 DNA topoisomerase -
FQ475_RS16185 24956..25241 - 286 Protein_31 recombinase family protein -
FQ475_RS16190 25296..25976 - 681 WP_001067799.1 IS6-like element IS1216 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia - 1..26633 26633
- inside IS/Tn erm(B) / aph(3')-III / ant(6)-Ia - 11618..19491 7873
- flank IS/Tn - - 25296..25976 680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32777.35 Da        Isoelectric Point: 4.7269

>T288203 WP_060763469.1 NZ_LR135422:c21108-20239 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT288203 WP_000205227.1 NZ_LR135422:c21347-21123 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References