Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 13653..14790 Replicon plasmid 4
Accession NZ_LR135422
Organism Enterococcus faecium isolate E8440

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag FQ475_RS16110 Protein ID WP_074394525.1
Coordinates 13653..14516 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL01
Locus tag FQ475_RS16115 Protein ID WP_002331065.1
Coordinates 14518..14790 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FQ475_RS16075 8894..9151 + 258 WP_002326781.1 hypothetical protein -
FQ475_RS16080 9403..9975 - 573 WP_000170424.1 recombinase family protein -
FQ475_RS16085 9991..10596 - 606 WP_000599739.1 Fic family protein -
FQ475_RS16090 10997..11566 - 570 Protein_12 FtsX-like permease family protein -
FQ475_RS16095 11618..12298 - 681 WP_001067799.1 IS6-like element IS1216 family transposase -
FQ475_RS16100 12445..12528 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
FQ475_RS16105 12653..13390 + 738 WP_002321849.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
FQ475_RS16110 13653..14516 - 864 WP_074394525.1 zeta toxin family protein Toxin
FQ475_RS16115 14518..14790 - 273 WP_002331065.1 antitoxin Antitoxin
FQ475_RS16120 14807..15016 - 210 WP_000527318.1 peptide-binding protein -
FQ475_RS16125 15115..16011 - 897 WP_002334978.1 AAA family ATPase -
FQ475_RS16130 16309..16881 - 573 WP_001079938.1 HTH domain-containing protein -
FQ475_RS16135 17157..17951 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
FQ475_RS16140 18044..18586 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
FQ475_RS16145 18583..19491 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia - 1..26633 26633
- inside IS/Tn erm(B) / aph(3')-III / ant(6)-Ia - 11618..19491 7873


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32611.17 Da        Isoelectric Point: 6.6627

>T288202 WP_074394525.1 NZ_LR135422:c14516-13653 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10733.17 Da        Isoelectric Point: 4.4865

>AT288202 WP_002331065.1 NZ_LR135422:c14790-14518 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZL55

References