Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 529199..529770 Replicon chromosome
Accession NZ_LR135419
Organism Enterococcus faecium isolate E8440

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag FQ475_RS02535 Protein ID WP_002286801.1
Coordinates 529429..529770 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag FQ475_RS02530 Protein ID WP_002323011.1
Coordinates 529199..529429 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FQ475_RS02505 524332..525663 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
FQ475_RS02510 525685..526311 + 627 WP_002286816.1 cysteine hydrolase -
FQ475_RS02515 526494..527075 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
FQ475_RS02520 527807..528369 + 563 Protein_500 SOS response-associated peptidase family protein -
FQ475_RS02525 528574..528912 - 339 WP_002286804.1 hypothetical protein -
FQ475_RS02530 529199..529429 + 231 WP_002323011.1 hypothetical protein Antitoxin
FQ475_RS02535 529429..529770 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FQ475_RS02540 530620..530805 + 186 WP_002304207.1 hypothetical protein -
FQ475_RS02545 531310..531504 + 195 WP_002297028.1 hypothetical protein -
FQ475_RS02550 531695..531944 + 250 Protein_506 transposase -
FQ475_RS02555 532188..533018 - 831 WP_002286789.1 manganese catalase family protein -
FQ475_RS02560 533226..534560 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T288199 WP_002286801.1 NZ_LR135419:529429-529770 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT288199 WP_002323011.1 NZ_LR135419:529199-529429 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References