Detailed information of TA system TA288194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 40589..41697
• Replicon: plasmid 3
• Accession: NZ_LR135410
• Organism: Enterococcus faecium isolate E8284

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: -
• Locus tag: EQB56_RS16690
• Protein ID: WP_060763469.1
• Coordinates: 40589..41458 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: EQB56_RS16695
• Protein ID: WP_000205227.1
• Coordinates: 41473..41697 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQB56_RS16660	36659..37231	-	573	WP_001079938.1	HTH domain-containing protein	-
EQB56_RS16670	37507..38301	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
EQB56_RS16675	38394..38936	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
EQB56_RS16680	38933..39841	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
EQB56_RS16685	39874..40608	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
EQB56_RS16690	40589..41458	-	870	WP_060763469.1	nucleotidyltransferase domain-containing protein	Toxin
EQB56_RS16695	41473..41697	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
EQB56_RS16700	41840..43402	-	1563	WP_000136908.1	recombinase family protein	-
EQB56_RS16705	43404..43820	-	417	WP_000323438.1	recombinase	-
EQB56_RS16710	43821..44117	-	297	WP_002360685.1	hypothetical protein	-
EQB56_RS16715	44629..45306	-	678	Protein_51	DNA topoisomerase	-
EQB56_RS16720	45322..45924	-	603	WP_001062586.1	recombinase family protein	-
EQB56_RS17110	45938..46108	-	171	WP_000713595.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	VanHBX / erm(B) / aph(3')-III / ant(6)-Ia	-	1..63798	63798
	-	inside	IScluster/Tn	VanHBX / erm(B) / aph(3')-III / ant(6)-Ia	-	22860..39841	16981

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32777.35 Da
• Isoelectric Point: 4.7269

>T288194 WP_060763469.1 NZ_LR135410:c41458-40589 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT288194 WP_000205227.1 NZ_LR135410:c41697-41473 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp