Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 34003..35140 Replicon plasmid 3
Accession NZ_LR135410
Organism Enterococcus faecium isolate E8284

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag EQB56_RS16635 Protein ID WP_074394525.1
Coordinates 34003..34866 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL01
Locus tag EQB56_RS16640 Protein ID WP_002331065.1
Coordinates 34868..35140 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB56_RS16605 30206..30325 - 120 Protein_31 recombinase family protein -
EQB56_RS16610 30341..30946 - 606 WP_000599739.1 cell filamentation protein -
EQB56_RS16615 31347..31964 - 618 WP_099124473.1 FtsX-like permease family protein -
EQB56_RS16620 31968..32648 - 681 WP_001067799.1 IS6-like element IS1216 family transposase -
EQB56_RS16625 32795..32878 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
EQB56_RS16630 33003..33740 + 738 WP_002321849.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
EQB56_RS16635 34003..34866 - 864 WP_074394525.1 zeta toxin family protein Toxin
EQB56_RS16640 34868..35140 - 273 WP_002331065.1 antitoxin Antitoxin
EQB56_RS16645 35157..35366 - 210 WP_000527318.1 peptide-binding protein -
EQB56_RS16650 35465..36361 - 897 WP_002334978.1 ParA family protein -
EQB56_RS16660 36659..37231 - 573 WP_001079938.1 HTH domain-containing protein -
EQB56_RS16670 37507..38301 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EQB56_RS16675 38394..38936 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
EQB56_RS16680 38933..39841 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid VanHBX / erm(B) / aph(3')-III / ant(6)-Ia - 1..63798 63798
- inside IScluster/Tn VanHBX / erm(B) / aph(3')-III / ant(6)-Ia - 22860..39841 16981


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32611.17 Da        Isoelectric Point: 6.6627

>T288193 WP_074394525.1 NZ_LR135410:c34866-34003 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10733.17 Da        Isoelectric Point: 4.4865

>AT288193 WP_002331065.1 NZ_LR135410:c35140-34868 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZL55

References