Detailed information of TA system TA028819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2103023..2103654
• Replicon: chromosome
• Accession: NC_020181
• Organism: Enterobacter aerogenes EA1509E

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1Q8YTV3
• Locus tag: EAEROS_RS10850
• Protein ID: WP_012542177.1
• Coordinates: 2103478..2103654 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EAEROS_RS10845
• Protein ID: WP_041165571.1
• Coordinates: 2103023..2103430 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAEROS_RS10800	2098963..2099508	-	546	WP_015367382.1	terminase small subunit	-
EAEROS_RS10810	2099802..2100221	-	420	WP_015367383.1	hypothetical protein	-
EAEROS_RS10815	2100221..2100547	-	327	WP_015367384.1	hypothetical protein	-
EAEROS_RS10820	2100719..2100946	+	228	WP_134874998.1	hypothetical protein	-
EAEROS_RS10830	2101626..2102102	-	477	WP_173390334.1	DUF2514 domain-containing protein	-
EAEROS_RS10835	2102135..2102668	-	534	WP_015367388.1	lysozyme	-
EAEROS_RS10840	2102670..2102918	-	249	WP_032706073.1	class II holin family protein	-
EAEROS_RS10845	2103023..2103430	-	408	WP_041165571.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EAEROS_RS10850	2103478..2103654	-	177	WP_012542177.1	type II toxin-antitoxin system HicA family toxin	Toxin
EAEROS_RS10855	2104022..2104624	-	603	WP_041165614.1	hypothetical protein	-
EAEROS_RS10860	2104638..2105669	-	1032	WP_015367392.1	DUF968 domain-containing protein	-
EAEROS_RS10865	2105669..2105872	-	204	WP_015367393.1	hypothetical protein	-
EAEROS_RS10870	2105869..2106261	-	393	WP_015367394.1	SOS-response repressor and protease LexA	-
EAEROS_RS10880	2106604..2106837	-	234	WP_015367395.1	DinI family protein	-
EAEROS_RS10885	2107031..2107828	-	798	WP_041165572.1	hypothetical protein	-
EAEROS_RS10890	2107838..2108032	-	195	WP_015367397.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2065709..2118574	52865

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-61)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6632.83 Da
• Isoelectric Point: 11.0174

>T28819 WP_012542177.1 NC_020181:c2103654-2103478 [Klebsiella aerogenes EA1509E]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Nucleotide

• Length: 177 bp

>T28819 NC_020181:c2103654-2103478 [Klebsiella aerogenes EA1509E]
GTGAAGCAAAGCGAGTTGAGACGTTGGCTTGCAGCTCAAGGGGCAGAGTTTAAAGATGGTACTAACCACCTGAAAATCTA
CCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTGAAGC
AACTTGGCATCAAATAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15020.03 Da
• Isoelectric Point: 4.4368

>AT28819 WP_041165571.1 NC_020181:c2103430-2103023 [Klebsiella aerogenes EA1509E]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSADGEAFVEVPSSVAAKVLLL
NRLVSTNTSNAELARMINTRPQEVQRIVSLGHSTKIDTIQKALAALGQHMEIVVR

Nucleotide

• Length: 408 bp

>AT28819 NC_020181:c2103430-2103023 [Klebsiella aerogenes EA1509E]
ATGCGATACCCGGTAATTTTTGAACATGATGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
CGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCCTGGTAACGGCGTTTGATTTCTACTTCGACGATCGCC
GGGAGATTCCCGCGCCATCAGCTGACGGTGAGGCATTTGTTGAGGTTCCGTCCAGCGTAGCGGCGAAGGTATTGCTGCTA
AATCGTCTGGTAAGCACCAACACCAGCAATGCTGAACTTGCCCGCATGATTAATACGCGTCCGCAGGAAGTACAGCGCAT
CGTATCGCTTGGTCACAGCACCAAAATTGATACGATTCAAAAAGCTCTGGCAGCGCTGGGACAGCATATGGAAATTGTCG
TTCGTTAA

Structures

Toxin

• AlphaFold DB: A0A1Q8YTV3