288189

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 59276..60384 Replicon plasmid 3
Accession NZ_LR135403
Organism Enterococcus faecium isolate E8377

Toxin (Protein)


Gene name MNTss Uniprot ID -
Locus tag EQB54_RS16815 Protein ID WP_060763469.1
Coordinates 59276..60145 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag EQB54_RS16820 Protein ID WP_000205227.1
Coordinates 60160..60384 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB54_RS16785 55346..55918 - 573 WP_001079938.1 HTH domain-containing protein -
EQB54_RS16795 56194..56988 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EQB54_RS16800 57081..57623 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
EQB54_RS16805 57620..58528 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB54_RS16810 58561..59295 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB54_RS16815 59276..60145 - 870 WP_060763469.1 nucleotidyltransferase domain-containing protein Toxin
EQB54_RS16820 60160..60384 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
EQB54_RS16825 60527..62089 - 1563 WP_000136908.1 recombinase family protein -
EQB54_RS16830 62091..62507 - 417 WP_000323438.1 recombinase -
EQB54_RS16835 62508..62804 - 297 WP_002360685.1 hypothetical protein -
EQB54_RS16840 63316..63798 - 483 Protein_73 DNA topoisomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid VanHBX / erm(B) / aph(3')-III / ant(6)-Ia - 1..63798 63798
- inside IScluster/Tn VanHBX / erm(B) / aph(3')-III / ant(6)-Ia - 41547..58528 16981


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32777.35 Da        Isoelectric Point: 4.7269

>T288189 WP_060763469.1 NZ_LR135403:c60145-59276 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT288189 WP_000205227.1 NZ_LR135403:c60384-60160 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References