Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 52690..53827 Replicon plasmid 3
Accession NZ_LR135403
Organism Enterococcus faecium isolate E8377

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag EQB54_RS16760 Protein ID WP_074394525.1
Coordinates 52690..53553 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL01
Locus tag EQB54_RS16765 Protein ID WP_002331065.1
Coordinates 53555..53827 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB54_RS16730 48893..49012 - 120 Protein_53 recombinase family protein -
EQB54_RS16735 49028..49633 - 606 WP_000599739.1 cell filamentation protein -
EQB54_RS16740 50034..50651 - 618 WP_099124473.1 FtsX-like permease family protein -
EQB54_RS16745 50655..51335 - 681 WP_001067799.1 IS6-like element IS1216 family transposase -
EQB54_RS16750 51482..51565 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
EQB54_RS16755 51690..52427 + 738 WP_002321849.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
EQB54_RS16760 52690..53553 - 864 WP_074394525.1 zeta toxin family protein Toxin
EQB54_RS16765 53555..53827 - 273 WP_002331065.1 antitoxin Antitoxin
EQB54_RS16770 53844..54053 - 210 WP_000527318.1 peptide-binding protein -
EQB54_RS16775 54152..55048 - 897 WP_002334978.1 ParA family protein -
EQB54_RS16785 55346..55918 - 573 WP_001079938.1 HTH domain-containing protein -
EQB54_RS16795 56194..56988 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EQB54_RS16800 57081..57623 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
EQB54_RS16805 57620..58528 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid VanHBX / erm(B) / aph(3')-III / ant(6)-Ia - 1..63798 63798
- inside IScluster/Tn VanHBX / erm(B) / aph(3')-III / ant(6)-Ia - 41547..58528 16981


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32611.17 Da        Isoelectric Point: 6.6627

>T288188 WP_074394525.1 NZ_LR135403:c53553-52690 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10733.17 Da        Isoelectric Point: 4.4865

>AT288188 WP_002331065.1 NZ_LR135403:c53827-53555 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZL55

References