Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 5177..6285 Replicon plasmid 3
Accession NZ_LR135366
Organism Enterococcus faecium isolate E8195

Toxin (Protein)


Gene name MNTss Uniprot ID -
Locus tag EQB49_RS15850 Protein ID WP_060763469.1
Coordinates 5177..6046 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag EQB49_RS15855 Protein ID WP_000205227.1
Coordinates 6061..6285 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB49_RS15820 1247..1819 - 573 WP_001079938.1 HTH domain-containing protein -
EQB49_RS15830 2095..2889 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EQB49_RS15835 2982..3524 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
EQB49_RS15840 3521..4429 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB49_RS15845 4462..5196 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB49_RS15850 5177..6046 - 870 WP_060763469.1 nucleotidyltransferase domain-containing protein Toxin
EQB49_RS15855 6061..6285 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
EQB49_RS15860 6428..7990 - 1563 WP_000136908.1 recombinase family protein -
EQB49_RS15865 7992..8408 - 417 WP_000323438.1 recombinase -
EQB49_RS15875 8831..9979 - 1149 WP_002287525.1 IS200/IS605 family element transposase accessory protein TnpB -
EQB49_RS15880 9996..10400 - 405 WP_002287522.1 IS200/IS605-like element ISEfa4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-III / ant(6)-Ia / erm(B) - 1..31867 31867
- inside IScluster/Tn aph(3')-III / ant(6)-Ia - 25..14735 14710


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32777.35 Da        Isoelectric Point: 4.7269

>T288169 WP_060763469.1 NZ_LR135366:c6046-5177 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT288169 WP_000205227.1 NZ_LR135366:c6285-6061 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References