Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 18235..19343 Replicon plasmid 3
Accession NZ_LR135353
Organism Enterococcus faecium isolate E8014

Toxin (Protein)


Gene name MNTss Uniprot ID R3JIN1
Locus tag EQB45_RS16200 Protein ID WP_000233000.1
Coordinates 18474..19343 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag EQB45_RS16195 Protein ID WP_000205227.1
Coordinates 18235..18459 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB45_RS16165 13501..14340 + 840 Protein_14 type IA DNA topoisomerase -
EQB45_RS16170 14417..15097 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EQB45_RS16175 15437..16606 - 1170 WP_002300493.1 IS256 family transposase -
EQB45_RS16180 16844..17092 + 249 Protein_17 Gfo/Idh/MocA family oxidoreductase -
EQB45_RS16185 17166..17846 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EQB45_RS16190 17901..18092 + 192 Protein_19 recombinase family protein -
EQB45_RS16195 18235..18459 + 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
EQB45_RS16200 18474..19343 + 870 WP_000233000.1 nucleotidyltransferase domain-containing protein Toxin
EQB45_RS16205 19324..20058 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB45_RS16210 20091..20999 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB45_RS16215 20996..21476 + 481 Protein_24 GNAT family N-acetyltransferase -
EQB45_RS16220 21621..21622 - 2 WP_164852608.1 IS6-like element IS1216 family transposase -
EQB45_RS16225 22452..22664 + 213 WP_002349227.1 hypothetical protein -
EQB45_RS16230 22826..23344 + 519 WP_000357244.1 YfbU family protein -
EQB45_RS16235 23351..23863 + 513 WP_000774078.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32926.57 Da        Isoelectric Point: 4.8781

>T288160 WP_000233000.1 NZ_LR135353:18474-19343 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT288160 WP_000205227.1 NZ_LR135353:18235-18459 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3JIN1

Antitoxin

Source ID Structure

References