Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 177724..178861 Replicon plasmid 2
Accession NZ_LR135352
Organism Enterococcus faecium isolate E8014

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag EQB45_RS15695 Protein ID WP_002332783.1
Coordinates 177724..178587 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag EQB45_RS15700 Protein ID WP_002326825.1
Coordinates 178589..178861 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB45_RS15660 173410..173982 - 573 WP_002300842.1 SIS domain-containing protein -
EQB45_RS15665 173989..174651 - 663 WP_002300841.1 ribulose-phosphate 3-epimerase -
EQB45_RS15670 174667..175182 - 516 WP_002300840.1 YjbQ family protein -
EQB45_RS15675 175184..175468 - 285 WP_002300838.1 PTS sugar transporter subunit IIB -
EQB45_RS15680 175502..176212 - 711 Protein_188 PTS galactitol transporter subunit IIC -
EQB45_RS15685 176266..176946 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EQB45_RS15690 177021..177161 - 141 Protein_190 IS6 family transposase -
EQB45_RS15695 177724..178587 - 864 WP_002332783.1 toxin zeta Toxin
EQB45_RS15700 178589..178861 - 273 WP_002326825.1 antitoxin Antitoxin
EQB45_RS15705 178879..179088 - 210 WP_000527318.1 peptide-binding protein -
EQB45_RS15710 179186..180082 - 897 WP_002326827.1 ParA family protein -
EQB45_RS15720 181604..181864 - 261 Protein_196 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
EQB45_RS15730 182034..182771 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
EQB45_RS16710 182896..182994 - 99 WP_176698583.1 23S rRNA methyltransferase attenuation leader peptide -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aac(6')-aph(2'') - 1..238961 238961
- inside IScluster/Tn erm(B) / aac(6')-aph(2'') - 176266..190347 14081


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T288158 WP_002332783.1 NZ_LR135352:c178587-177724 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT288158 WP_002326825.1 NZ_LR135352:c178861-178589 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References