Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 18448..19585 Replicon plasmid 4
Accession NZ_LR135342
Organism Enterococcus faecium isolate E7356

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag EQB70_RS16570 Protein ID WP_074394525.1
Coordinates 18722..19585 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL01
Locus tag EQB70_RS16565 Protein ID WP_002331065.1
Coordinates 18448..18720 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB70_RS16525 13747..14655 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB70_RS16530 14652..15194 + 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
EQB70_RS16535 15287..16081 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EQB70_RS16545 16357..16929 + 573 WP_001079938.1 HTH domain-containing protein -
EQB70_RS16555 17227..18123 + 897 WP_002334978.1 ParA family protein -
EQB70_RS16560 18222..18431 + 210 WP_000527318.1 peptide-binding protein -
EQB70_RS16565 18448..18720 + 273 WP_002331065.1 antitoxin Antitoxin
EQB70_RS16570 18722..19585 + 864 WP_074394525.1 zeta toxin family protein Toxin
EQB70_RS16575 19848..20585 - 738 WP_002321849.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
EQB70_RS16580 20710..20793 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
EQB70_RS16585 20940..21620 + 681 WP_001067799.1 IS6-like element IS1216 family transposase -
EQB70_RS16590 21624..22241 + 618 WP_099124473.1 FtsX-like permease family protein -
EQB70_RS16595 22642..23247 + 606 WP_000599739.1 cell filamentation protein -
EQB70_RS16600 23263..23835 + 573 WP_000170424.1 recombinase family protein -
EQB70_RS16605 24087..24344 - 258 WP_002326781.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(6)-Ia / aph(3')-III / erm(B) - 1..28557 28557
- inside IS/Tn ant(6)-Ia / aph(3')-III / erm(B) - 13747..21620 7873


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32611.17 Da        Isoelectric Point: 6.6627

>T288151 WP_074394525.1 NZ_LR135342:18722-19585 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10733.17 Da        Isoelectric Point: 4.4865

>AT288151 WP_002331065.1 NZ_LR135342:18448-18720 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZL55

References