Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 11891..12999 Replicon plasmid 4
Accession NZ_LR135342
Organism Enterococcus faecium isolate E7356

Toxin (Protein)


Gene name MNTss Uniprot ID -
Locus tag EQB70_RS16515 Protein ID WP_060763469.1
Coordinates 12130..12999 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag EQB70_RS16510 Protein ID WP_000205227.1
Coordinates 11891..12115 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB70_RS16900 7480..7650 + 171 WP_000713595.1 hypothetical protein -
EQB70_RS16485 7664..8266 + 603 WP_001062586.1 recombinase family protein -
EQB70_RS16490 8282..8959 + 678 Protein_10 DNA topoisomerase -
EQB70_RS16495 9471..9767 + 297 WP_002360685.1 hypothetical protein -
EQB70_RS16500 9768..10184 + 417 WP_000323438.1 recombinase -
EQB70_RS16505 10186..11748 + 1563 WP_000136908.1 recombinase family protein -
EQB70_RS16510 11891..12115 + 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
EQB70_RS16515 12130..12999 + 870 WP_060763469.1 nucleotidyltransferase domain-containing protein Toxin
EQB70_RS16520 12980..13714 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB70_RS16525 13747..14655 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB70_RS16530 14652..15194 + 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
EQB70_RS16535 15287..16081 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EQB70_RS16545 16357..16929 + 573 WP_001079938.1 HTH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(6)-Ia / aph(3')-III / erm(B) - 1..28557 28557
- inside IS/Tn ant(6)-Ia / aph(3')-III / erm(B) - 13747..21620 7873


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32777.35 Da        Isoelectric Point: 4.7269

>T288150 WP_060763469.1 NZ_LR135342:12130-12999 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT288150 WP_000205227.1 NZ_LR135342:11891-12115 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References