Detailed information of TA system TA288150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 11891..12999
• Replicon: plasmid 4
• Accession: NZ_LR135342
• Organism: Enterococcus faecium isolate E7356

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: -
• Locus tag: EQB70_RS16515
• Protein ID: WP_060763469.1
• Coordinates: 12130..12999 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: EQB70_RS16510
• Protein ID: WP_000205227.1
• Coordinates: 11891..12115 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQB70_RS16900	7480..7650	+	171	WP_000713595.1	hypothetical protein	-
EQB70_RS16485	7664..8266	+	603	WP_001062586.1	recombinase family protein	-
EQB70_RS16490	8282..8959	+	678	Protein_10	DNA topoisomerase	-
EQB70_RS16495	9471..9767	+	297	WP_002360685.1	hypothetical protein	-
EQB70_RS16500	9768..10184	+	417	WP_000323438.1	recombinase	-
EQB70_RS16505	10186..11748	+	1563	WP_000136908.1	recombinase family protein	-
EQB70_RS16510	11891..12115	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
EQB70_RS16515	12130..12999	+	870	WP_060763469.1	nucleotidyltransferase domain-containing protein	Toxin
EQB70_RS16520	12980..13714	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
EQB70_RS16525	13747..14655	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
EQB70_RS16530	14652..15194	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
EQB70_RS16535	15287..16081	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
EQB70_RS16545	16357..16929	+	573	WP_001079938.1	HTH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ant(6)-Ia / aph(3')-III / erm(B)	-	1..28557	28557
	-	inside	IS/Tn	ant(6)-Ia / aph(3')-III / erm(B)	-	13747..21620	7873

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32777.35 Da
• Isoelectric Point: 4.7269

>T288150 WP_060763469.1 NZ_LR135342:12130-12999 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT288150 WP_000205227.1 NZ_LR135342:11891-12115 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp