Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 33813..34921 Replicon plasmid 4
Accession NZ_LR135327
Organism Enterococcus faecium isolate E7654

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag EQB39_RS15920 Protein ID WP_002303392.1
Coordinates 33813..34682 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag EQB39_RS15925 Protein ID WP_002303393.1
Coordinates 34697..34921 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB39_RS15890 29109..29360 - 252 Protein_36 GNAT family N-acetyltransferase -
EQB39_RS15895 29605..30900 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
EQB39_RS15900 31001..31909 + 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
EQB39_RS15905 31912..32160 - 249 WP_002347175.1 hypothetical protein -
EQB39_RS15910 32157..33065 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB39_RS15915 33098..33832 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB39_RS15920 33813..34682 - 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
EQB39_RS15925 34697..34921 - 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
EQB39_RS15930 35064..35165 - 102 Protein_44 site-specific recombinase -
EQB39_RS15935 35307..36581 + 1275 WP_002398627.1 ISL3 family transposase -
EQB39_RS15940 36630..37394 - 765 Protein_46 recombinase zinc beta ribbon domain-containing protein -
EQB39_RS15945 38082..38885 - 804 Protein_47 lincosamide nucleotidyltransferase Lnu(B) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid lsa(E) / tet(L) / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) - 1..38929 38929
- inside IScluster/Tn lsa(E) / tet(L) / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) - 10..38885 38875


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T288143 WP_002303392.1 NZ_LR135327:c34682-33813 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT288143 WP_002303393.1 NZ_LR135327:c34921-34697 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References