288142

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 25335..26472 Replicon plasmid 4
Accession NZ_LR135327
Organism Enterococcus faecium isolate E7654

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag EQB39_RS15865 Protein ID WP_001284311.1
Coordinates 25609..26472 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag EQB39_RS15860 Protein ID WP_000301765.1
Coordinates 25335..25607 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB39_RS15830 21022..22398 - 1377 WP_001574277.1 tetracycline efflux MFS transporter Tet(L) -
EQB39_RS15835 22432..22482 - 51 Protein_25 tetracycline resistance efflux system leader peptide -
EQB39_RS15840 22592..23956 - 1365 Protein_26 TetM/TetW/TetO/TetS family tetracycline resistance ribosomal protection protein -
EQB39_RS15845 24018..24698 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EQB39_RS15850 24754..25011 + 258 WP_002347171.1 hypothetical protein -
EQB39_RS15855 25109..25318 + 210 WP_000527318.1 peptide-binding protein -
EQB39_RS15860 25335..25607 + 273 WP_000301765.1 antitoxin Antitoxin
EQB39_RS15865 25609..26472 + 864 WP_001284311.1 toxin zeta Toxin
EQB39_RS15870 26735..27472 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
EQB39_RS15875 27597..27680 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
EQB39_RS15880 27729..27812 - 84 Protein_34 peptide-binding protein -
EQB39_RS15885 28222..29016 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EQB39_RS15890 29109..29360 - 252 Protein_36 GNAT family N-acetyltransferase -
EQB39_RS15895 29605..30900 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid lsa(E) / tet(L) / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) - 1..38929 38929
- inside IScluster/Tn lsa(E) / tet(L) / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) - 10..38885 38875


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T288142 WP_001284311.1 NZ_LR135327:25609-26472 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT288142 WP_000301765.1 NZ_LR135327:25335-25607 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References