288137

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 22410..23518 Replicon plasmid 4
Accession NZ_LR135320
Organism Enterococcus faecium isolate E7663

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag EQB37_RS15570 Protein ID WP_002303392.1
Coordinates 22410..23279 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag EQB37_RS15575 Protein ID WP_002303393.1
Coordinates 23294..23518 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB37_RS15540 17706..17957 - 252 Protein_21 GNAT family N-acetyltransferase -
EQB37_RS15545 18202..19497 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
EQB37_RS15550 19598..20506 + 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
EQB37_RS15555 20509..20757 - 249 WP_002347175.1 hypothetical protein -
EQB37_RS15560 20754..21662 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB37_RS15565 21695..22429 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB37_RS15570 22410..23279 - 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
EQB37_RS15575 23294..23518 - 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
EQB37_RS15580 23661..23762 - 102 Protein_29 site-specific recombinase -
EQB37_RS15585 23904..25178 + 1275 WP_002398627.1 ISL3 family transposase -
EQB37_RS15590 25227..25991 - 765 Protein_31 recombinase zinc beta ribbon domain-containing protein -
EQB37_RS15595 26679..27482 - 804 Protein_32 lincosamide nucleotidyltransferase Lnu(B) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(L) / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 1..38929 38929
- inside IScluster/Tn tet(L) / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 3290..37645 34355


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T288137 WP_002303392.1 NZ_LR135320:c23279-22410 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT288137 WP_002303393.1 NZ_LR135320:c23518-23294 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References