288136

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 13932..15069 Replicon plasmid 4
Accession NZ_LR135320
Organism Enterococcus faecium isolate E7663

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag EQB37_RS15515 Protein ID WP_001284311.1
Coordinates 14206..15069 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag EQB37_RS15510 Protein ID WP_000301765.1
Coordinates 13932..14204 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB37_RS15480 9619..10995 - 1377 WP_001574277.1 tetracycline efflux MFS transporter Tet(L) -
EQB37_RS15485 11029..11079 - 51 Protein_10 tetracycline resistance efflux system leader peptide -
EQB37_RS15490 11189..12553 - 1365 Protein_11 TetM/TetW/TetO/TetS family tetracycline resistance ribosomal protection protein -
EQB37_RS15495 12615..13295 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EQB37_RS15500 13351..13608 + 258 WP_002347171.1 hypothetical protein -
EQB37_RS15505 13706..13915 + 210 WP_000527318.1 peptide-binding protein -
EQB37_RS15510 13932..14204 + 273 WP_000301765.1 antitoxin Antitoxin
EQB37_RS15515 14206..15069 + 864 WP_001284311.1 toxin zeta Toxin
EQB37_RS15520 15332..16069 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
EQB37_RS15525 16194..16277 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
EQB37_RS15530 16326..16409 - 84 Protein_19 peptide-binding protein -
EQB37_RS15535 16819..17613 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EQB37_RS15540 17706..17957 - 252 Protein_21 GNAT family N-acetyltransferase -
EQB37_RS15545 18202..19497 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(L) / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 1..38929 38929
- inside IScluster/Tn tet(L) / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 3290..37645 34355


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T288136 WP_001284311.1 NZ_LR135320:14206-15069 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT288136 WP_000301765.1 NZ_LR135320:13932-14204 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References