Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 6269..7377 Replicon plasmid 5
Accession NZ_LR135311
Organism Enterococcus faecium isolate E7240

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag EQB75_RS16980 Protein ID WP_002303392.1
Coordinates 6269..7138 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag EQB75_RS16985 Protein ID WP_002303393.1
Coordinates 7153..7377 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB75_RS16950 1565..1816 - 252 Protein_3 GNAT family N-acetyltransferase -
EQB75_RS16955 2061..3356 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
EQB75_RS16960 3457..4365 + 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
EQB75_RS16965 4368..4616 - 249 WP_002347175.1 hypothetical protein -
EQB75_RS16970 4613..5521 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB75_RS16975 5554..6288 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB75_RS16980 6269..7138 - 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
EQB75_RS16985 7153..7377 - 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
EQB75_RS16990 7520..8341 - 822 Protein_11 recombinase zinc beta ribbon domain-containing protein -
EQB75_RS16995 9029..9517 - 489 Protein_12 lincosamide nucleotidyltransferase Lnu(B) -
EQB75_RS17000 9572..10252 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EQB75_RS17005 10559..11077 + 519 WP_000357244.1 YfbU family protein -
EQB75_RS17010 11084..11596 + 513 WP_000774078.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-III / ant(6)-Ia / erm(B) - 1..27647 27647
- inside IScluster/Tn aph(3')-III / ant(6)-Ia / erm(B) - 678..27575 26897


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T288131 WP_002303392.1 NZ_LR135311:c7138-6269 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT288131 WP_002303393.1 NZ_LR135311:c7377-7153 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References