Detailed information of TA system TA288122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 231239..231908
• Replicon: plasmid 2
• Accession: NZ_LR135294
• Organism: Enterococcus faecium isolate E7237

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: EQB69_RS15105
• Protein ID: WP_010708525.1
• Coordinates: 231239..231634 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL01
• Locus tag: EQB69_RS15110
• Protein ID: WP_002331065.1
• Coordinates: 231636..231908 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQB69_RS15070	226970..227698	-	729	WP_010726886.1	hypothetical protein	-
EQB69_RS15075	227958..228506	-	549	WP_002292681.1	hypothetical protein	-
EQB69_RS15080	228507..229364	-	858	WP_002292680.1	AAA family ATPase	-
EQB69_RS15085	229650..229937	-	288	WP_002300557.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EQB69_RS15090	229927..230256	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
EQB69_RS15095	230333..230479	-	147	Protein_239	Txe/YoeB family addiction module toxin	-
EQB69_RS15100	230531..231211	-	681	Protein_240	IS6 family transposase	-
EQB69_RS15105	231239..231634	-	396	WP_010708525.1	zeta toxin family protein	Toxin
EQB69_RS15110	231636..231908	-	273	WP_002331065.1	antitoxin	Antitoxin
EQB69_RS15115	231925..232134	-	210	WP_000527318.1	peptide-binding protein	-
EQB69_RS15120	232233..233129	-	897	WP_002334978.1	ParA family protein	-
EQB69_RS15125	233373..234668	+	1296	WP_002326809.1	ISL3-like element ISEfa5 family transposase	-
EQB69_RS15135	234949..235521	-	573	WP_001079938.1	HTH domain-containing protein	-
EQB69_RS15145	235797..236591	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / aac(6')-aph(2'') / aph(3')-III	-	1..256017	256017
	-	inside	IScluster/Tn	aph(3')-III	-	225349..241632	16283

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-118)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14867.86 Da
• Isoelectric Point: 5.2505

>T288122 WP_010708525.1 NZ_LR135294:c231634-231239 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGFCCKVLNLLSNKVE

Nucleotide

• Length: 396 bp

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10733.17 Da
• Isoelectric Point: 4.4865

>AT288122 WP_002331065.1 NZ_LR135294:c231908-231636 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Nucleotide

• Length: 273 bp

Structures

Antitoxin

• AlphaFold DB: A0A828ZL55