Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 4690..5827 Replicon plasmid 6
Accession NZ_LR135283
Organism Enterococcus faecium isolate E6975

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag EQB65_RS17240 Protein ID WP_001284311.1
Coordinates 4690..5553 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag EQB65_RS17245 Protein ID WP_000301765.1
Coordinates 5555..5827 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB65_RS17200 1..945 + 945 WP_002303559.1 replication initiation protein -
EQB65_RS17205 966..1067 + 102 Protein_1 HTH domain-containing protein -
EQB65_RS17210 1142..1822 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EQB65_RS17215 1877..2053 + 177 Protein_3 GNAT family N-acetyltransferase -
EQB65_RS17220 2146..2940 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EQB65_RS17225 3350..3433 + 84 Protein_5 peptide-binding protein -
EQB65_RS17230 3482..3565 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
EQB65_RS17235 3690..4427 + 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
EQB65_RS17240 4690..5553 - 864 WP_001284311.1 toxin zeta Toxin
EQB65_RS17245 5555..5827 - 273 WP_000301765.1 antitoxin Antitoxin
EQB65_RS17250 5844..6053 - 210 WP_000527318.1 peptide-binding protein -
EQB65_RS17255 6218..6898 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EQB65_RS17260 6964..7416 + 453 Protein_12 DUF536 domain-containing protein -
EQB65_RS17575 7431..7601 + 171 WP_032491833.1 hypothetical protein -
EQB65_RS17265 8056..8280 + 225 WP_002363584.1 bacteriocin hiracin-JM79 -
EQB65_RS17270 8300..8587 + 288 WP_002363589.1 bacteriocin immunity protein -
EQB65_RS17280 9037..9417 + 381 WP_010782660.1 MobC family plasmid mobilization relaxosome protein -
EQB65_RS17285 9399..10313 + 915 WP_010782659.1 relaxase/mobilization nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-III / erm(B) - 1..12090 12090
- inside IScluster/Tn aph(3')-III / erm(B) - 1136..6904 5768


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T288114 WP_001284311.1 NZ_LR135283:c5553-4690 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT288114 WP_000301765.1 NZ_LR135283:c5827-5555 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References